Synthesis, crystal structure and DNA binding studies of a binuclear copper(II) complex with phenanthroline.
Synthesis, crystal structure and DNA binding studies of a binuclear copper(II) complex with phenanthroline.
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DOI：
10.1016/j.jinorgbio.2006.03.010
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发表时间：
2006-08
影响因子：
3.9
通讯作者：
Qian-qian Zhang;Fang Zhang;Wei-guo Wang;Xiu-lin Wang
中科院分区：
文献类型：
--
作者：
Qian-qian Zhang;Fang Zhang;Wei-guo Wang;Xiu-lin Wang
合成并表征了一种新的双核铜配合物[Cu_2Ph_2Cl_4]（Phen= 1，10-菲咯啉）。单晶X射线衍射结果表明该配合物属于单斜晶系，Cc（no. 9），晶胞尺寸：a=9.849（2），B=17.833（4），c=13.374（3），β=106.61（3）°，V=2251.0（8）3，Dc=1.8569 Mgm−3，F（000）=1256.0，Z=4。一个Cu（II）中心原子位于扭曲的正方形平面几何是四配位的。另一个位于扭曲的正方形金字塔几何结构中，是五配位的。配合物中只有一个桥连氯原子。通过电子吸收光谱、荧光光谱、电导率测量和荧光激发-发射三向数据阵列的并行因子分析（PARAFAC）研究了配合物与DNA的结合行为。结果表明，配合物与DNA的摩尔比较低时（最大为0.3），DNA二级结构发生断裂，而摩尔比较高时，DNA发生插入。此外，用PARAFAC算法可以直接得到EB-DNA和EB（EB：溴化乙锭）的平衡浓度，是研究复合物与DNA相互作用的有效方法。
A new binuclear copper(II) complex, [Cu2Phen2Cl4] (Phen=1,10-phenanthroline), has been synthesized and characterized. Single crystal X-ray diffraction results suggest that this complex structure belongs to monoclinic crystal system, Cc (no. 9) with the cell dimensions: a=9.849(2)Å, b=17.833(4)Å, c=13.374(3)Å, β=106.61(3)°, V=2251.0(8)Å3, Dc=1.8569 Mgm−3, F(000)=1256.0, Z=4. One Cu(II) central atom situated in a distorted square planar geometry is four-coordinated. The other situated in a distorted square pyramidal geometry is five-coordinated. Only one bridging Cl atom exists in the complex. Spectroscopic studies, including electronic absorption and fluorescence spectra, conductivity measurements and parallel factor analysis (PARAFAC) of fluorescence excitation–emission three-way data array, were carried out on the DNA binding behavior of the complex. All the results suggested that the breakage of DNA secondary structure took place at low molar ratio of complex to DNA (0.3 at most) and intercalation into the base pair of DNA took place at high molar ratio. Additionally, the equilibrium concentration of EB–DNA and EB (EB: ethidium bromide) could be directly obtained by PARAFAC algorithm, proved to be a convincing method for studying the interaction of complexes with DNA.