Comparison of DNA histograms by standard flow cytometry and image cytometry on sections in Barrett's adenocarcinoma.
Comparison of DNA histograms by standard flow cytometry and image cytometry on sections in Barrett's adenocarcinoma.
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DOI：
10.1186/1472-6890-8-5
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发表时间：
2008-05-30
影响因子：
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通讯作者：
Goyal RK
中科院分区：
文献类型：
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作者：
Huang Q;Yu C;Zhang X;Goyal RK
本研究的目的是比较通过标准流式细胞术 (FC) 和高保真图像细胞术切片 (ICS) 在正常胃肠粘膜和巴雷特腺癌 (BAC) 中获得的 DNA 直方图。检查了来自 10 名受试者的 10 名正常对照的存档福尔马林固定石蜡包埋组织块和来自 17 名患者的 42 份 BAC 组织。 DNA FC 使用标准技术进行，ICS 通过自动细胞成像系统 (ACIS) 进行。 DNA 倍性直方图分为峰值 DNA 指数 (DI) 在 0.9-1.1 的二倍体和峰值 DI > 1.1 的非整倍体。测定非整倍体峰的 DI 值。此外，对于 DNA ICS，还鉴定了代表 DNA 含量异质性的异质性指数 (HI)，以及包含 DI > G2 的细胞的直方图。通过 FC 和 ICS 分析，所有对照样品均为二倍体。在 BAC 中，FC 在 29% 的样品中显示二倍体峰，在 57% 和 14% 的样品中分别显示二倍体峰和额外的非整倍体或四倍体峰。相比之下，ICS 在所有病例中都显示出非整倍体峰，峰值 DI > 1.25； 37例峰值DI在1.25至2.25之间； 5例峰值DI>2.25。对照组的 HI 值（平均值±标准差）为 11.3 ± 1.1，BAC 为 32.4 ± 8.5（p < 0.05）。对照没有G2超过细胞。然而，主峰 DI < 2.25 的病例中有 19/37 (51%) 的细胞超过 9N。 ICS 检测到所有 BAC 样本中的 DNA 非整倍体，而 FC 则漏掉了 29% 的非整倍体诊断。此外，ICS 还提供有关 HI 和 G2 超出率的更多信息。
The purpose of this study was to compare DNA histograms obtained by standard flow cytometry (FC) and high fidelity image cytometry on sections (ICS) in normal gastrointestinal mucosa and Barrett's adenocarcinoma (BAC). Archival formalin-fixed paraffin-embedded tissue blocks of 10 normal controls from 10 subjects and 42 BAC tissues from 17 patients were examined. DNA FC was performed using standard techniques and ICS was carried out by Automated Cellular Imaging System (ACIS). DNA ploidy histograms were classified into diploid with peak DNA index (DI) at 0.9–1.1, and aneuploid with peak DI > 1.1. DI values of aneuploid peaks were determined. Additionally, for DNA ICS, heterogeneity index (HI) representing DNA content heterogeneity, and histograms containing cells with DI > G2 were also identified. All control samples were diploid by both FC and ICS analyses. In BAC, FC showed diploid peaks in 29%, diploid peaks with additional aneuploid or tetraploid peaks in 57%, and 14% of the samples, respectively. In contrast, ICS showed aneuploid peaks in all the cases with peak DI > 1.25; 37 cases had peak DI between 1.25 and 2.25; and 5 cases had peak DI > 2.25. HI values (mean ± SD) were 11.3 ± 1.1 in controls and 32.4 ± 8.5 in BAC (p < 0.05). Controls had no G2 exceeding cells. However, 19/37 (51%) of the cases with primary peak DI < 2.25 had cells exceeding 9N. ICS detects DNA aneuploidy in all BAC samples while FC missed the diagnosis of aneuploidy in 29%. In addition, ICS provides more information on HI and G2 exceeding rates.