HISTOLOGICAL FEATURES OF FLUOROSED HUMAN DENTAL ENAMEL
HISTOLOGICAL FEATURES OF FLUOROSED HUMAN DENTAL ENAMEL
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DOI：
10.1159/000260157
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发表时间：
1975-01-01
期刊：
影响因子：
4.2
通讯作者：
MOLLER, IJ
中科院分区：
文献类型：
--
作者：
FEJERSKOV, O;SILVERSTONE, LM;MOLLER, IJ
本研究描述了轻度或重度氟中毒牙齿牙釉质的组织学特征。在水性介质或醇和喹啉中吸收后，在透射光和偏振光下检查切片。为了进行半定量研究，准备了等值线图。此外，沿着牙釉质的横断面并在选定的点进行定量吸入研究。根据总光程差的值，计算双折射并绘制吸液图。最后，制作了接触式显微放射线照片。研究表明，只有在严重氟斑牙病例中，才有可能将组织学变化程度与临床特征相关联。氟牙釉质的一般形态与正常牙釉质没有不同，但组织包含弥漫性低矿化区域。与显微射线照相术相反，在偏振光下，观察到了更广泛的变化。因此，仅在使用偏振光时才记录到轻微程度的氟斑牙。在严重的情况下，记录到的最大孔隙度为 25% 或更高，并且位于紧邻矿化良好的牙釉质表面的深处。在严重的氟中毒病例中，在喹啉中检查时仅观察到暗区，定量吸入研究表明，这些病例中牙釉质的反应与最初龋齿病变中所描述的反应相似。在轻微和严重的氟斑牙中，表面下的多孔区域在水性介质中表现为“混合体”，但在酒精中检查时，它们只能在严重的情况下完全穿透组织。因此，在轻度氟斑牙中，地下区域表现为“分子筛”。本研究的结果与之前对牙釉质形成的观察结果相关，以解释氟斑牙的发病机制。
The present study describes the histological features of enamel in teeth showing either mild or severe fluorosis. Sections were examined in transmitted and polarized light after imbibition in aqueous media or alcohols and quinoline. For semiquantitative study contour maps were prepared. Furthermore quantitative imbibition studies were performed along a traverse through the enamel and at selected points. From the values of total path difference, birefringence was calculated and imbibition graphs drawn. Finally, contact microradiographs were made. The study showed that it is only possible to correlate the degree of histological change with clinical features in severe cases of dental fluorosis. The general morphology of fluorosed enamel does not differ from normal enamel, but the tissue comprise areas of diffuse hypomineralization. In polarized light, opposite to microradiography, much more extensive changes were observed. Thus, slight degrees of dental fluorosis were recorded only when using polarized light. In severe cases, a maximum degree of 25% porosity or more were recorded and located immediately deep to a well-mineralized enamel surface. In severe cases of fluorosis, only dark zones were observed when examined in quinoline, and the quantitative imbibition studies showed that the enamel in these cases reacted similar to what is described in initial caries lesions. In both slight and severe dental fluorosis the subsurface porous region behaved as a ‘mixed body’ in aqueous media, but when examined in alcohols, these could only penetrate the tissue completely in severe cases. Thus, in mild dental fluorosis the subsurface region behaved as a ‘molecular sieve’. The findings in the present study are correlated to previous observations of enamel formation in order to explain the pathogenesis of dental fluorosis.