Large Fecal Reservoir of Escherichia coli Sequence Type 131-H30 Subclone Strains That Are Shared Within Households and Resemble Clinical ST131-H30 Isolates
Large Fecal Reservoir of Escherichia coli Sequence Type 131-H30 Subclone Strains That Are Shared Within Households and Resemble Clinical ST131-H30 Isolates
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DOI：
10.1093/infdis/jiz669
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发表时间：
2020-05-15
影响因子：
6.4
通讯作者：
Johnson, James R.
中科院分区：
文献类型：
--
作者：
Mohamed, Muhanad;Clabots, Connie;Johnson, James R.
背景新出现的耐抗生素大肠埃希菌主要代表序列类型131（ST 131）内的嵌套（氟喹诺酮耐药[FQR]）H30 R和H30 Rx亚克隆。肠道定植和家庭内传播可能是H30 R出现的基础。我们从741名志愿者（383名退伍军人，358名家庭成员，包括宠物）的粪便样本中筛选ST 131和FQR E。大肠杆菌（ESTREC）中，并使用分子谱分析来解析独特的菌株。选择的菌株进行基于PCR的毒力基因组、序列类型（ST）、H30、H30 Rx和53个毒力基因（VG）的检测。家庭内的菌株共享进行了比较，家庭，主机和细菌特性。粪便分离株与临床分离株进行比较。H30 R的定植率为5.1%，ST 131为8%（67%为ST 131），ST 131为10%（52%为ST 131）。ST 131分离株比非ST 131分离株表现出更多的VG。菌株共享（27%的多受试者家庭，18%的相应受试者）与老年人、ESTREC、H30 R、H30 Rx、ST 73和特定VG相关。根据所有研究的特征，粪便ST 131和EscherichREC分离株与同期和历史临床分离株相似。退伍军人和他们的人类家庭成员通常携带并广泛分享ST 131，主要是H30 R，从而可能促进ST 131大流行。菌株共享对应于多种细菌特征，包括FQ抗性和特异性VG，这可能促进肠道定植和/或宿主-宿主传播。
Background. Emerging antimicrobial-resistant Escherichia coli represent mainly the nested (fluoroquinolone-resistant [FQR]) H30R and H30Rx subclones within sequence type 131 (ST131). Intestinal colonization and within-household transmission may underlie H30R's emergence.Methods. We screened fecal samples from 741 volunteers (383 veterans, 358 household members, including pets) for ST131 and FQR E. coli (FQREC) and used molecular profiling to resolve unique strains. Selected strains underwent PCR-based detection of phylogroups, sequence types (STs), H30, H30Rx, and 53 virulence genes (VGs). Within-household strain sharing was compared with household, host, and bacterial characteristics. Fecal isolates were compared with clinical isolates.Results. Colonization prevalence was 5.1% for H30R, 8% for ST131 (67% FQREC), and 10% for FQREC (52% ST131). ST131 isolates exhibited more VGs than non-ST131 isolates. Strain sharing (27% of multisubject households, 18% of corresponding subjects) was associated with the elderly, FQREC, H30R, H30Rx, ST73, and specific VGs. Fecal ST131 and FQREC isolates resembled contemporaneous and historical clinical isolates according to all studied traits.Conclusions. Veterans and their human household members commonly carry and extensively share FQREC, predominantly H30R, thereby likely facilitating the ST131 pandemic. Strain sharing corresponds with multiple bacterial characteristics, including FQ resistance and specific VGs, which may promote intestinal colonization and/or host-to-host transmission.