A polyclonal antiserum against the rabbit progesterone receptor recognizes the human receptor: biochemical characterization.
A polyclonal antiserum against the rabbit progesterone receptor recognizes the human receptor: biochemical characterization.
复制标题
针对兔孕酮受体的多克隆抗血清可识别人类受体：生化特征。
DOI：
10.1002/cbf.290070210
复制
发表时间：
1989
影响因子：
3.6
通讯作者：
Satyaswaroop,PG
中科院分区：
文献类型：
--
作者：
Feil,PD;Clarke,CL;Satyaswaroop,PG
在用 116 000 只 Mrrabbit 子宫孕激素受体 (PR) 免疫的豚鼠中产生多克隆抗血清。 PR抗原通过DEAE-纤维素层析和制备型十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行部分纯化，转移到硝化纤维素上，然后将116 000个Mrband切除并注射到豚鼠中。抗血清在蛋白质印迹上识别出 Mr116 000 和 81 000 的兔子宫 PR。根据蔗糖梯度上的沉降位移、免疫沉淀研究、蛋白质印迹和使用光标记样品的荧光分析确定，抗血清对正常和恶性人体组织的 PR 具有特异性。用这种多克隆抗血清或最近获得的人 PR 单克隆抗体探测的蛋白质印迹的比较表明，两种抗血清在兔和人样品中识别出相似的 PR 结构。多克隆抗血清的表征已证明其适用于研究正常和恶性人体组织中的免疫定位或 PR 以及在蛋白质印迹上检测到的受体结构。
Polyclonal antiserum was generated in guinea pigs immunized with the 116 000 Mrrabbit uterine progesterone receptor (PR). The PR antigen was partially purified by DEAE‐cellulose chromatography and preparative sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred to nitrocellulose, and the 116 000 Mrband excised and injected into guinea pigs. The antiserum recognized on protein blots rabbit uterine PR of Mr116 000 and 81 000. The antiserum was judged to be specific for PR from normal and malignant human tissues as determined by sedimentation shift on sucrose gradients, immunoprecipitation studies, protein blotting, and fluorographic analysis using photolabelled samples. Comparison of protein blots probed with this polyclonal antiserum or with a recently obtained monoclonal antibody to human PR indicated that similar PR structures were recognized in rabbit and human samples by both antisera. Characterization of the polyclonal antiserum has demonstrated its suitability for investigating the immunolocalization or PR in normal and malignant human tissues as well as the receptor structure detected on protein blots.