MUTANTS OF CHLAMYDOMONAS-MOEWUSII WITH IMPAIRED MOTILITY
MUTANTS OF CHLAMYDOMONAS-MOEWUSII WITH IMPAIRED MOTILITY
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DOI：
10.1099/00221287-11-3-358
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发表时间：
1954-01-01
期刊：
影响因子：
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通讯作者：
LEWIN, RA
中科院分区：
文献类型：
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作者：
LEWIN, RA
利用紫外辐照和特殊富集技术，获得了13个具有麻痹性鞭毛的突变体。两种交配型中有11种是通过与野生型杂交获得的。M. 1060（阴性）未引起性行为。其余12个突变体未检测到等位基因。在M. 1002 +中，麻痹位点似乎与交配型位点密切相关。在某些突变体中，当瘫痪的正配子与野生型负配子配对时，在几个小时后恢复了运动能力。恢复归因于运动因子通过连接配对配子的原生质桥。当与不同突变株的麻痹阳性细胞配对时，麻痹阴性细胞可作为这些因子的供体。由遗传上不同的配子交配形成的配对被认为是异核体，它可以用来提供与瘫痪相关的基因的同一性或非同一性的信息。在正常鞭毛活动的一些传播因素的作用，因此牵连。
The object of these investigations was to apply physiological genetics to the study of flagellar movement Thirteen mutants with paralyzed flagella were obtained by the use of UV irradiation and a special enrichment technique. Eleven of these were obtained in both mating types by crossing with wild type. No sexual activity was elicited in M. 1060 (minus). No allelism was detected among the remaining 12 mutants. In M. 1002 plus, the locus for paralysis appears to be very closely linked with that of mating type. In certain mutants, paralyzed plus gametes, when paired with wild-type minus, recovered motility after some hours. Recovery is attributed to the passage of motility factors across the protoplasmic bridge linking the paired gametes. Paralyzed minus cells may act as donors of such factors when paired with paralyzed plus cells of an unlike mutant strain. The pairs formed by mating genetically dissimilar gametes are considered as heterokaryons, which can be used to provide information on the identity or non-identity of genes associated with paralysis. The role of a number of transmissible factors in normal flagellar activity is thereby implicated.