Vancomycin-modified nanoparticles for efficient targeting and preconcentration of Gram-positive and Gram-negative bacteria
Vancomycin-modified nanoparticles for efficient targeting and preconcentration of Gram-positive and Gram-negative bacteria
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DOI：
10.1021/nn700183g
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发表时间：
2008-09-01
期刊：
影响因子：
17.1
通讯作者：
Simard, Benoit
中科院分区：
文献类型：
--
作者：
Kell, Arnold J.;Stewart, Gale;Simard, Benoit
开发了一系列万古霉素修饰的纳米颗粒，并将其用于磁约束实验，从水溶液中分离出多种革兰氏阳性和革兰氏阴性细菌。我们确定万古霉素在纳米颗粒表面的取向/结构和整体表面覆盖率对于介导纳米颗粒与病原体细胞壁表面之间快速有效的相互作用至关重要，并且在一系列修饰的纳米颗粒中，只有一种取向/结构才能有效和可重复地捕获几种重要的致病菌。有趣的是，随着纳米颗粒直径的增加（从类似的50纳米到2800纳米），为了使表面结合探针有效地限制革兰氏阳性细菌，有必要在纳米颗粒表面和万古霉素部分之间加入一个长连接体。最后，我们还确定，随着纳米颗粒的尺寸和纳米颗粒上万古霉素表面覆盖率的增加，有效标记和随后的磁约束所需的时间显着减少。随着疾病检测技术向基于“芯片实验室”的平台过渡，有必要制定策略，有效且廉价地将细胞从大体积预先浓缩到更适合这些类型的微流体设备的体积。这些小分子修饰的超顺磁性纳米颗粒可以提供一种实现这一目标的方法。
A series of vancomycin-modified nanoparticles were developed and employed in magnetic confinement assays to isolate a variety of Gram-positive and Gram-negative bacteria from aqueous solution. We determined that the orientation/architecture of vancomycin on the surface of the nanoparticles and the overall surface coverage is critical in mediating fast and effective interactions between the nanoparticle and the pathogen cell wall surface and only one orientation/architecture in a series of modified nanoparticles leads to the efficient and reproducible capture of several important pathogenic bacteria. Interestingly, as the nanoparticles increase in diameter (from similar to 50 to 2800 nm), it is necessary to incorporate a long linker between the nanoparticle surface and the vancomycin moiety in order for the surface bound probe to efficiently confine Gram-positive bacteria. Finally, we also determined that the time required for efficient labeling and subsequent magnetic confinement significantly decreases as the size of the nanoparticle and the vancomycin surface coverage on the nanoparticle increases. As disease detection technologies transition to "lab-on-a-chip" based platforms it is necessary to develop strategies to effectively and inexpensively preconcentrate cells from large volume to volumes more amenable to these types of microfluidic devices. These small molecule-modified superparamagnetic nanoparticles can provide a means by which this can be accomplished.