Force Measurements for Cancer Cells.
Force Measurements for Cancer Cells.
复制标题
癌细胞的力测量。
DOI：
10.1007/978-1-4939-6646-2_12
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Farrell,Brenda
中科院分区：
文献类型：
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作者：
Rajasekharan,Vivek;Sreenivasan,VarunKA;Farrell,Brenda
在细胞骨架重塑过程中，癌细胞在质膜上产生源自化学马达（例如肌动蛋白）的力。该力 (pN) 及其时间进程反映了电机的开启和关闭速率。我们描述了测力装置（即光镊）的设计和校准，该装置用于监测细胞边缘的力及其时间过程，特别强调仪器的时间分辨率。
During cytoskeleton remodeling, cancer cells generate force at the plasma membrane that originates from chemical motors (e.g., actin). This force (pN) and its time course reflect the on and off-rates of the motors. We describe the design and calibration of a force-measuring device (i.e., optical tweezers) that is used to monitor this force and its time course at the edge of a cell, with particular emphasis on the temporal resolution of the instrument.