Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
复制标题
DOI：
10.1016/j.cyto.2009.06.009
复制
发表时间：
2009-09
期刊：
影响因子：
3.8
通讯作者：
Yang Sun;Dinghua Yi;Yunya Wang;Renhong Zheng;Guocheng Sun;Jing Wang;Yang Liu;Jun Ren;Yuemin Wang;Shumiao Zhang;Chunhu Gu;Jianming Pei
中科院分区：
文献类型：
--
作者：
Yang Sun;Dinghua Yi;Yunya Wang;Renhong Zheng;Guocheng Sun;Jing Wang;Yang Liu;Jun Ren;Yuemin Wang;Shumiao Zhang;Chunhu Gu;Jianming Pei
本研究旨在探讨年龄对婴幼儿先天性心脏病手术后循环内皮祖细胞（EPCs）及其动员的影响。对60例婴幼儿（1个月~ 3岁）行房/室间隔缺损修补术，分别于术前、体外循环结束时、术后6、12、24、48、72、96 h测定EPCs数量及血浆IL-6、IL-8、IL-10、TNF-α、VEGF、G-CSF水平。术前EPCs随着年龄的增加而减少，与血浆VEGF和G-CSF水平的变化相似。体外循环心脏手术可诱导EPCs和血浆VEGF、G-CSF的快速动员，且EPCs、VEGF、G-CSF的增加量随年龄的增长而减少。IL-6、IL-8、IL-10和TNF-α在不同年龄组的升高幅度相似。6个月以下婴儿EPCs、血浆VEGF和G-CSF的动员能力有限，与炎性IL活化的变化无关。术前EPCs和血浆VEGF和G-CSF水平随着年龄的增加而降低。尽管在接受体外循环的婴儿中观察到EPCs显着增加和细胞趋化因子的释放，但<6个月大的婴儿EPCs的动员有限。
This study was designed to find the effects of age on circulating endothelial progenitor cells (EPCs) and their mobilization in infants and young children following surgical correction of congenital heart defects. In 60 consecutive infants and young children (1month to 3years old) undergoing repair of atrial/ventricular septal defect, the numbers of EPCs and plasma levels of IL-6, -8, -10, TNF-α, VEGF and G-CSF were determined preoperatively, at the end of cardiopulmonary bypass (CPB), as well as 6, 12, 24, 48, 72 and 96h following surgery. Preoperative EPCs were reduced with increased age, similar to changes in plasma VEGF and G-CSF levels. Rapid mobilizations of EPCs and plasma VEGF, G-CSF were induced by cardiac surgery with CPB in all infants and young children, and the increased volumes of EPCs, VEGF and G-CSF decreased with age decreasing. The increased volumes of IL-6, -8, -10 and TNF-α were similar in different age groups. However, mobilization of EPCs, plasma VEGF and G-CSF were limited in infants <6months old, which did not correlate with change in inflammatory IL activation. Preoperative EPCs and plasma levels of VEGF and G-CSF were reduced with increasing age in infants and young children. Although a significant increase in EPCs and release of cytochemokines were observed in infants undergoing CPB, the mobilization of EPCs of the infants <6months old are limited.