Vertical spread of neuronal activity within the cat motor cortex investigated with epicortical stimulation and intracellular recording.
Vertical spread of neuronal activity within the cat motor cortex investigated with epicortical stimulation and intracellular recording.
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通过皮层刺激和细胞内记录研究猫运动皮层内神经元活动的垂直传播。
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发表时间：
1985
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通讯作者：
Tomokazu Oshima
中科院分区：
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作者：
K. Ezure;M. Oguri;Tomokazu Oshima
在脑隔离猫制剂中，前交叉皮层的表面受到电刺激。记录刺激部位下方的细胞内反应，以评估活动在皮质层上的垂直分布。对于调整强度以在直接皮质反应（DCR）中引起纯负波的外皮层刺激（EPICS），只有相对浅层的一些神经元以兴奋性突触后电位（EPSP）做出反应。刺激强度增强，可引起所有测试细胞 EPSP、抑制性突触后电位 (IPSP) 或两者中产生的 DCR 负波和随后的正波。很少观察到细胞的直接或轴突逆向激发。具有 EPSP 的细胞分布在所有层中，在层 II 和 V-VI 中有两个峰值群体。 IPSPs主要位于第三层的上半部，也有少数位于较浅层和较深层。 EPSP 和 IPSP 均表现出单突触潜伏期或寡突触潜伏期（0.6-10 毫秒），其在深层往往比表层更长。一些深层细胞，包括快锥体细胞和慢锥体束细胞，显示出树突起源的缓慢上升的单突触 EPSP。进一步的晚期反应包括 EPSP、IPSP、功能障碍 (DF) 和去抑制 (DI)。 DF或DI发生在一些深层细胞中。假定了两种活动垂直传播模式：一种是级联传输，增加了向深处的反应能力，另一种是 EPSP 沿树突的电紧张传播。
In the encéphale isolé cat preparation the surface of precruciate cortex was electrically stimulated. Intracellular responses underneath the stimulated site were recorded to assess the vertical spread of activities across the cortical layers. To the epicortical stimulation (EPICS) with intensity adjusted to evoke a pure negative wave in the direct cortical response (DCR), only some neurons in relatively superficial layers responded with excitatory postsynaptic potentials (EPSPs). Stimuli intensified to evoke both the negative and subsequent positive waves in DCR produced in all tested cells either EPSPs, inhibitory postsynaptic potentials (IPSPs), or both. Direct or axonal antidromic excitation of the cell was observed only infrequently. Cells with EPSPs distributed through all the layers with two peak populations in laminae II and V-VI. Those with IPSPs were located mainly in the upper half of lamina III with a few in more superficial as well as in deeper layers. Both EPSPs and IPSPs showed mono- or oligosynaptic latencies (0.6-10 msec) that tended to become longer in deep than in superficial layers. Some deep layer cells including fast and slow pyramidal tract cells showed slowly rising monosynaptic EPSPs of dendritic origin. Further late responses consisted of EPSPs, IPSPs, disfacilitation (DF), and disinhibition (DI). DF or DI occurred in some deep layer cells. Two modes of vertical spread of activities were postulated: one the cascade transmissions which increased response repertoire toward the depths, and the other the electrotonic spread of EPSPs along dendrites.