Omega-3 fatty acid regulates inflammatory cytokine/mediator messenger RNA expression in Porphyromonas gingivalis-induced experimental periodontal disease
Omega-3 fatty acid regulates inflammatory cytokine/mediator messenger RNA expression in Porphyromonas gingivalis-induced experimental periodontal disease
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DOI：
10.1111/j.1399-302x.2007.00346.x
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发表时间：
2007-08-01
影响因子：
--
通讯作者：
Ebersole, J. L.
中科院分区：
文献类型：
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作者：
Kesavalu, L.;Bakthavatchalu, V.;Ebersole, J. L.
简介：牙龈卟啉单胞菌通过诱导炎性细胞因子，导致牙龈和牙周组织炎症和牙槽骨吸收，与成人牙周炎的病因学密切相关。这项研究测试了假设，即补充饮食与ω-3脂肪酸（ω-3 FA;即鱼油）将发挥抗炎作用的牙龈卟啉单胞菌感染rates.Methods的牙龈组织：大鼠喂鱼油或玉米油饮食随意22周，感染牙龈卟啉单胞菌菌株381或菌株A7 A1 -28。处死后，切除大鼠牙龈组织，分离RNA并分析促炎介质[白细胞介素-1 β（IL-1 β）、肿瘤坏死因子-α（TNF-α）、IL-6]、T辅助细胞1型和2型细胞因子[干扰素-γ（IFN-γ），IL-4，IL-10），抗氧化酶[过氧化氢酶（CAT），超氧化物歧化酶（SOD）]，和类花生酸介质产生的关键基因[环加氧酶-2（考克斯-2），结果：与喂食玉米油饮食的大鼠相比，omega-3 FA饮食的大鼠表现出降低的促炎细胞因子基因表达（IL-1 β，TNF-α）和增强的IFN-γ，CAT和SOD信使RNA表达，支持饮食诱导的宿主炎症反应的调节。对大鼠牙槽骨吸收相关基因表达谱的分析表明，IL-1 β、IL-6和考克斯-2与牙槽骨吸收相关，而CAT和SOD与牙槽骨吸收相关。结论：富含omega-3 FA的饮食可调节口腔牙龈卟啉单胞菌感染后宿主局部牙龈炎症环境，从而影响大鼠牙槽骨吸收。
Introduction: Porphyromonas gingivalis is strongly implicated in the etiology of adult periodontitis by inducing inflammatory cytokines, resulting in gingival and periodontal tissue inflammation and alveolar bone resorption. This study tested the hypothesis that supplementing the diet with omega-3 fatty acid (omega-3 FA; i.e. fish oil) would exert anti-inflammatory effects in the gingival tissues of P. gingivalis-infected rats.Methods: Rats were fed either fish oil or corn oil diets ad libitum for 22 weeks and infected with P. gingivalis strain 381 or strain A7A1-28. After sacrifice, rat gingival tissues were excised and the RNA was isolated and analyzed for proinflammatory mediators [interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), IL-6], T helper type 1 and type 2 cytokines [interferon-gamma (IFN-gamma), IL-4, IL-10), antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD)], and genes critical for eicosanoid mediator production [cyclo-oxygenase-2 (COX-2), 5-lipoxygenase (5-LO)] by reverse transcription-polymerase chain reaction using rat-specific primers.Results: Rats on the omega-3 FA diet exhibited decreased proinflammatory cytokine gene expression (IL-1 beta, TNF-alpha) and enhanced IFN-gamma, CAT and SOD messenger RNA expression compared to rats fed a corn oil diet, supporting a diet-induced modulation of host inflammatory reactions. Analyses of alveolar bone resorption in the rats related to gene expression profiles demonstrated significant positive correlations with IL-1 beta, IL-6 and COX-2 and negative correlations with CAT and SOD.Conclusion: These findings suggest that diets enriched for omega-3 FA modulate the local gingival inflammatory milieu of the host following oral P. gingivalis infection, which impacts on alveolar bone resorption in rats.