[Model for immunologic testing of biomaterials].
[Model for immunologic testing of biomaterials].
复制标题
[生物材料免疫检测模型]。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
E. Karle
中科院分区：
文献类型：
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作者：
R. Thull,;K. Trautner;E. Karle
腐蚀产物和电场能够将蛋白质转变为抗原，从而允许免疫系统将生物材料识别为外来物。腐蚀产物和大分子之间的反应也导致抗原（载体抗原），例如由植入物表面处的电场引起的大分子（例如蛋白质）的构象变化（改性大分子抗原）。虽然对腐蚀的敏感性和原电池的有效性可通过电化学方法测量，但确定生物材料表面附近的场强的合适方法仍然不可用。双层的未涂覆和涂覆的钛表面上的蛋白质的构象和它们转化为抗原的影响进行了研究与多克隆抗体能够识别不变的蛋白质，尽管吸附到表面。14C标记的牛血清白蛋白用作模型蛋白。通过检测发射的电子来确定吸附的蛋白质分子总数。天然蛋白质浓度与吸附分子浓度的商给出了生物相容性指数，该指数与表面积无关，并给出了材料预期生物相容性的指示。在动物实验中证实了钛和钛上两种涂层材料的生物学试验结果。在未来，免疫学试验有可能取代动物实验。
Corrosion products and electric fields are capable of changing proteins to antigens, thus permitting the immunological system to identify the biomaterial as foreign. The reaction between corrosion products and a macro-molecule also leads to an antigen (carrier antigen), such as conformational changes of a macro-molecule, e.g. a protein, caused by the electric field at the implant surface (modified macro-molecule antigen). While the sensitivity to corrosion and the effectiveness of galvanic elements is measurable by electrochemical methods, suitable methods of determining the field strength in the vicinity of biomaterial surfaces are still unavailable. The influence of the double layer of uncoated and coated titanium surfaces on the conformation of proteins and their conversion to antigens are investigated with polyclonal antibodies capable of identifying the unchanged protein despite adsorption to the surface. 14C-marked Bovine Serum Albumin serves as a model protein. Determination of the total number of protein molecules adsorbed is effected via the detection of the emitted electrons. The quotient of the concentration of natural proteins to the concentration of adsorbed molecules gives the biocompatibility index, which is independent of the surface area, and gives an indication of the expected biocompatibility of the material. The results of the biological tests of titanium and two coating materials on titanium were confirmed in an animal experiment. It is possible that in the future immunological tests may replace experiments in animals.