Cloning, expression, and chromosomal localization of human long-chain fatty acid CoA ligase 4 (FACL4)
Cloning, expression, and chromosomal localization of human long-chain fatty acid CoA ligase 4 (FACL4)
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DOI：
10.1006/geno.1998.5268
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发表时间：
1998-04-15
期刊：
影响因子：
4.4
通讯作者：
Prescott, SM
中科院分区：
文献类型：
--
作者：
Cao, Y;Traer, E;Prescott, SM
长链脂肪酸-CoA连接酶（也称为脂肪酸酰基-CoA合成酶）在脂质生物合成和脂肪酸降解中起重要作用。我们在此报告的cDNA克隆的人长链脂肪酸辅酶A连接酶4从大脑文库。该cDNA编码一种功能性长链脂肪酸-CoA连接酶，该酶对花生四烯酸具有偏好性。我们还通过北方杂交研究了基因表达的组织分布，人胎盘、脑、睾丸、卵巢、脾和肾上腺皮质具有最高水平的长链脂肪酸-CoA连接酶4，而GI系统具有最低水平。经FISH分析，该基因定位于人类染色体Xq 23。(C)北京：科学出版社.
Long-chain fatty acid-CoA ligase (also called fatty acid acyl-CoA synthetase) plays an essential role in lipid biosynthesis and fatty acid degradation. We report herein the cDNA cloning of the human long-chain fatty acid-CoA ligase 4 from a brain library. The cDNA encodes a functional long-chain fatty acid-CoA ligase that shows preference for arachidonic acid as substrate, We also studied the tissue distribution of gene expression by Northern hybridization, Human placenta, brain, testis, ovary, spleen, and adrenal cortex have the highest levels of expression of the long-chain fatty acid-CoA ligase 4, whereas the GI system has the lowest, Finally, this gene was localized to chromosome Xq23 in human by FISH analysis. (C) 1998 Academic Press.