Protein aggregation in Escherichia coli: role of proteases.
Protein aggregation in Escherichia coli: role of proteases.
复制标题
大肠杆菌中的蛋白质聚集：蛋白酶的作用。
DOI：
10.1111/j.1574-6968.2002.tb11020.x
复制
发表时间：
2002
影响因子：
2.1
通讯作者：
E. Ron
中科院分区：
文献类型：
--
作者：
R. Rosen;D. Biran;Eyal Gur;D. Becher;M. Hecker;E. Ron
蛋白质聚集与多种人类疾病有关，并且被认为是蛋白质质量控制的重要过程。在细菌中，蛋白质在应激条件下发生聚集，例如热休克。我们研究了热休克期间大肠杆菌的蛋白质聚集体。我们的结果表明，聚集体中蛋白质的浓度和多样性取决于蛋白酶的可用性。从 Lon (La) 蛋白酶突变体获得的聚集体含有比野生型聚集体多三倍的蛋白质，并显示出最广泛的蛋白质多样性。结果支持这样的假设：蛋白质聚集体是由部分未折叠的蛋白质形成的，这些蛋白质未被分子伴侣重折叠或被蛋白酶降解。
Protein aggregation is involved in several human diseases, and presumed to be an important process in protein quality control. In bacteria, aggregation of proteins occurs during stress conditions, such as heat shock. We studied the protein aggregates of Escherichia coli during heat shock. Our results demonstrate that the concentration and diversity of proteins in the aggregates depend on the availability of proteases. Aggregates obtained from mutants in the Lon (La) protease contain three times more protein than wild-type aggregates and show the broadest protein diversity. The results support the assumption that protein aggregates are formed from partially unfolded proteins that were not refolded by chaperones or degraded by proteases.