An Improved Acute Gouty Arthritis Rat Model and Therapeutic Effect of Rhizoma Dioscoreae Nipponicae on Acute Gouty Arthritis Based on the Protein-Chip Methods
An Improved Acute Gouty Arthritis Rat Model and Therapeutic Effect of Rhizoma Dioscoreae Nipponicae on Acute Gouty Arthritis Based on the Protein-Chip Methods
复制标题
基于蛋白质芯片方法的改良急性痛风性关节炎大鼠模型及山药对急性痛风性关节炎的治疗作用
DOI：
10.1142/s0192415x12500103
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发表时间：
2012-01-01
影响因子：
5.7
通讯作者：
Liu, Shumin
中科院分区：
文献类型：
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作者：
Yao, Li;Dong, Wanru;Liu, Shumin
日本薯蓣（Dioscoreae Nipponicae， RDN）是一种中草药。在中医理论中，RDN的功能是祛风除湿。炎症机制在急性痛风性关节炎（AGA）的病理过程和预后中起重要作用。本研究的目的是通过对AGA大鼠滑膜蛋白的检测来确定其特异性表达蛋白。用尿酸钠晶体（MSU）联合次黄嘌呤（HX）建立AGA动物模型。献祭前采集血液样本用于测定血清尿酸。通过组织染色和蛋白定量评估结果（注射后2天）。采用RayBio (R) Human Label-based Antibody Array I芯片对AGA大鼠滑膜组织中的90种蛋白进行检测。在AGA大鼠滑膜中发现了14种不同表达的蛋白，其中9种是首次在该模型中发现。有7个上调和7个下调的蛋白，TRAIL和Neuropilin-2都可以被确定为AGA病理机制的关键贡献者。
Rhizoma Dioscoreae Nipponicae (RDN) is an herbal medicine. In the theories of Traditional Chinese Medicine (TCM), the function of RDN is to expel wind and remove dampness. Inflammatory mechanisms play an important role in the pathological process and prognosis of acute gouty arthritis (AGA). The aim of this study was to determine the specially expressed proteins through testing the proteins of the synovium in rats with AGA. The animal model of AGA was set up by Monosodium urate crystal (MSU) combined with hypoxanthine (HX), which was ameliorated in our previous experiment. Blood samples for measurement of serum uric acid were collected prior to sacrifice. Outcomes were assessed (two days after injection) by histological stain and protein quantitation. Three chips of RayBio (R) Human Label-based Antibody Array I were applied to detect 90 proteins in the synovium tissue of AGA rats. 14 differently expressed proteins were found in the synovium of AGA rats, and nine of them were first found in this model. There were seven up-regulated and seven down-regulated proteins, both TRAIL and Neuropilin-2 could be identified as key contributors to the pathomechanism of AGA.