Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
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DOI：
10.1007/s11064-011-0568-1
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发表时间：
2011-11-01
影响因子：
4.4
通讯作者：
Zablocka, Barbara
中科院分区：
文献类型：
--
作者：
Kawalec, Maria;Kowalczyk, Joanna E.;Zablocka, Barbara
Biphalin 是一种二聚阿片肽，对三种阿片受体（MOP、DOP 和 KOP）具有亲和力。 Biphalin 正在进行深入的临床前研究。人们认识到，δ-阿片受体的激活可引起针对脑缺氧和缺血的神经保护作用。我们比较了 Biphalin 和吗啡以及纳曲酮对阿片受体的抑制对 NMDA 攻击的大鼠器官型海马培养物中神经元存活的影响。研究结果：(1) 0.025-0.1 mu M biphalin 可减少 NMDA 诱导的神经元损伤； (2)双啡林神经保护作用被纳曲酮取消； (3) 即使在 NMDA 攻击后延迟应用 Biphalin，死亡细胞数量也会减少。
Biphalin is a dimeric opioid peptide that exhibits affinity for three types of opioid receptors (MOP, DOP and KOP). Biphalin is undergoing intensive preclinical study. It was recognized that activation of delta-opioid receptor elicits neuroprotection against brain hypoxia and ischemia. We compare the effect of biphalin and morphine and the inhibition of opioid receptors by naltrexone on survival of neurons in rat organotypic hippocampal cultures challenged with NMDA. Findings: (1) 0.025-0.1 mu M biphalin reduces NMDA-induced neuronal damage; (2) biphalin neuroprotection is abolished by naltrexone; (3) reduced number of dead cells is shown even if biphalin is applied with delay after NMDA challenge.