Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells.
Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells.
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DOI：
10.1210/endo.136.2.7835314
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发表时间：
1995-02
期刊：
影响因子：
4.8
通讯作者：
E. Jimi;T. Shuto;T. Koga
中科院分区：
文献类型：
--
作者：
E. Jimi;T. Shuto;T. Koga
当小鼠骨髓细胞与基质细胞系共培养时。ST 2时，大量破骨细胞样多核细胞（MNCs）形成。为了富集抗酒石酸酸性磷酸酶（TRAP）阳性的MNC，我们用分散酶处理培养物。将粘附于培养皿底部的富集的TRAP阳性MNC用含有15%胎牛血清的培养基再培养24小时。在培养过程中，超过80%的TRAP阳性MNCs从培养皿中脱落。然而，当在培养期间包括ST 2细胞时，TRAP阳性MNC的存活率显著增加。此外，在检测TRAP阳性MNC存活的各种因素中，仅M-CSF和IL-1 α可有效延长TRAP阳性MNC的存活率。
When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.