The Ras1-Cdc42 pathway is involved in hyphal development of Schizosaccharomyces japonicus
The Ras1-Cdc42 pathway is involved in hyphal development of Schizosaccharomyces japonicus
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DOI：
10.1093/femsyr/foy031
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发表时间：
2018-06-01
影响因子：
3.2
通讯作者：
Niki, Hironori
中科院分区：
文献类型：
--
作者：
Nozaki, Shingo;Furuya, Kanji;Niki, Hironori
双形酵母菌在环境的刺激下转变成丝状细胞或菌丝。二型酵母菌菌丝转变的机制主要在白色念珠菌（一种人类机会致病真菌）中进行了研究。Ras 1-MAPK信号通路是丝裂霉素C菌丝转变的主要信号转导途径。白色念珠菌。最近，非致病性的双态酵母SchizoschioschiomycesEscherichia coli也已被用于菌丝诱导的遗传分析。我们证实Sz. - 是的为了研究环境胁迫触发的信号转导是如何诱导菌丝转变的，我们研究了Sz中MAPK途径缺失突变体的菌丝转变。- 是的我们发现，丝裂原活化蛋白激酶途径不参与菌丝诱导，虽然交配反应是依赖于这些途径。然而，只有Ras 1缺失导致了严重的缺陷，菌丝发育通过DNA损伤和环境压力。事实上，Ras 1（Ras 1-Cdc 42）途径的Cdc 42分支上的基因efc 25 s1、scd 1 si和scd 2si是菌丝发育所需的。细胞形态学分析表明，Ras 1-Cdc 42途径缺失突变体菌丝顶端生长受到抑制。因此，Ras 1-Cdc 42途径对细胞极性的控制对于菌丝发育至关重要。
Dimorphic yeasts transform into filamentous cells or hyphae in response to environmental cues. The mechanisms for the hyphal transition of dimorphic yeasts have mainly been studied in Candida albicans, an opportunistic human fungal pathogen. The Ras1-MAPK pathway is a major signal transduction pathway for hyphal transition in C. albicans. Recently, the non-pathogenic dimorphic yeast Schizosaccharomyces japonicus has also been used for genetic analyses of hyphal induction. We confirmed that Ras1-MAPK and other MAPK pathways exist in Sz. japonicus. To examine how hyphal transition is induced by environmental stress-triggered signal transduction, we studied the hyphal transition of deletion mutants of MAPK pathways in Sz. japonicus. We found that the MAPK pathways are not involved in hyphal induction, although the mating response is dependent on these pathways. However, only Ras1 deletion caused a severe defect in hyphal development via both DNA damage and environmental stressors. In fact, genes on the Cdc42 branch of the Ras1 (Ras1-Cdc42) pathway, efc25s 1 , scd1si and scd2si, are required for hyphal development. Cell morphology analysis indicated that the apical growth of hyphal cells was inhibited in Ras1-Cdc42-pathway deletion mutants. Thus, the control of cell polarity by the Ras1-Cdc42 pathway is crucial for hyphal development.