DETECTION OF VIRAL-DNA IN NEONATAL HERPES-SIMPLEX VIRUS-INFECTIONS - FREQUENT AND PROLONGED PRESENCE IN SERUM AND CEREBROSPINAL-FLUID
DETECTION OF VIRAL-DNA IN NEONATAL HERPES-SIMPLEX VIRUS-INFECTIONS - FREQUENT AND PROLONGED PRESENCE IN SERUM AND CEREBROSPINAL-FLUID
复制标题
DOI：
10.1093/infdis/164.2.289
复制
发表时间：
1991-08-01
影响因子：
6.4
通讯作者：
MORISHIMA, T
中科院分区：
文献类型：
--
作者：
KIMURA, H;FUTAMURA, M;MORISHIMA, T
应用聚合酶链反应（PCR）技术检测7例新生儿口腔、皮肤、血清、脑脊液中单纯疱疹病毒（HSV）DNA。在培养阴性的患者中，通过检测HSV DNA证实了诊断。系列检查显示，HSV DNA在治疗开始后1-2周内仍保留在几名患者的血清和/或CSF中。然后，将新生儿HSV感染的PCR检测结果与单纯疱疹病毒性脑炎（HSE）的大龄儿童进行比较。从4名中枢神经系统受累的新生儿和所有9名HSE患儿的CSF中检测到HSV DNA。7名新生儿中有5名血清HSV DNA阳性，其中包括2例局部感染，但HSE患儿均为阴性。这些结果表明，HSV可能主要通过病毒血症在新生儿中传播。PCR检测可用于新生儿HSV感染的确诊，尤其是培养阴性的病例。
Polymerase chain reaction (PCR) assay was used to detect herpes simplex virus (HSV) DNA in mouth, skin, sera, or cerebrospinal fluid (CSF) from seven neonates with HSV infection. In a culture-negative patient, the diagnosis was confirmed by detection of HSV DNA. Serial examinations revealed that HSV DNA remained in the serum and/or CSF from several patients for 1-2 weeks after the beginning of treatment. Next, the results of PCR assay in neonatal HSV infections were compared with those in older children with herpes simplex encephalitis (HSE). HSV DNA was detected in CSF from four neonates with central nervous system involvement and in CSF from all nine children with HSE. Sera were positive for HSV DNA in five of seven neonates, including two cases of localized infections, but in none of the children with HSE. These results suggest that HSV may be spread principally via viremia in neonates. PCR assay could be useful for the confirmative diagnosis of neonatal HSV infections, especially in culture-negative cases.