Enhanced Recognition and Neutralization of HIV-1 by Antibody-Derived CCR5-Mimetic Peptide Variants
Enhanced Recognition and Neutralization of HIV-1 by Antibody-Derived CCR5-Mimetic Peptide Variants
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DOI：
10.1128/jvi.00967-12
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发表时间：
2012-11-01
影响因子：
5.4
通讯作者：
Farzan, Michael
中科院分区：
文献类型：
--
作者：
Chiang, Jessica J.;Gardner, Matthew R.;Farzan, Michael
酪氨酸硫酸化的CCR 5模拟肽CCR 5 mim 1比基于CCR 5氨基末端的磺基肽更有效地抑制HIV-1感染。在这里，我们的特点是CCR 5 mim 1和抗体PG 16的重链CDR 3的磺肽嵌合体。两个嵌合体结合了一系列包膜糖蛋白，中和HIV-1比CCR 5 mim 1更有效。其中之一的免疫粘附素形式CCR 5 mim 2-IG与CD 4-IG协同作用以中和HIV-1。这些磺肽是迄今为止描述的最广泛和最有效的CCR 5模拟肽之一。
A tyrosine-sulfated CCR5-mimetic peptide, CCR5mim1, inhibits HIV-1 infection more efficiently than sulfopeptides based on the CCR5 amino terminus. Here we characterized sulfopeptide chimeras of CCR5mim1 and the heavy-chain CDR3 of the antibody PG16. Two chimeras bound a range of envelope glycoproteins and neutralized HIV-1 more efficiently than CCR5mim1. An immunoadhesin form of one of these, CCR5mim2-Ig, synergized with CD4-Ig to neutralize HIV-1. These sulfopeptides are among the broadest and most potent CCR5-mimetic peptides described to date.