Splicing goes global
Splicing goes global
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DOI：
10.1016/s0168-9525(03)00091-x
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发表时间：
2003-06-01
影响因子：
11.4
通讯作者：
Beggs, JD
中科院分区：
文献类型：
--
作者：
Barrass, JD;Beggs, JD
转录学是对从细胞基因组转录的mRNAs的互补进行分析，目前主要关注成熟的、经过处理的mRNAs。然而，初级转录本的转录后处理可以显著影响成熟mRNAs的数量和结构，从而影响蛋白质产品的数量和结构。最近，内含子特异微阵列的发展使得对酿酒酵母中所有含内含子转录本的剪接进行了初步分析。在这里，我们讨论这些发现以及从这种方法中可能学到的东西。
Transcriptomics, the analysis of the complement of mRNAs transcribed from a cell's genome, currently focuses mainly on mature, processed mRNAs. However, posttranscriptional processing of primary transcripts can significantly affect both the quantity and the structure of the mature mRNAs and therefore of the protein products. Recently, the development of an intron-specific microarray has permitted a preliminary analysis of the splicing of all intron-containing transcripts in Saccharomyces cerevisiae. Here, we discuss the findings and what might be learned from this kind of approach.