DEMONSTRATION AND PARTIAL CHARACTERIZATION OF AN INTERMEDIATE HBCAG (DANE PARTICLE) POPULATION
DEMONSTRATION AND PARTIAL CHARACTERIZATION OF AN INTERMEDIATE HBCAG (DANE PARTICLE) POPULATION
复制标题
DOI：
10.1002/jmv.1890070309
复制
发表时间：
1981-01-01
影响因子：
12.7
通讯作者：
MEYER, KH
中科院分区：
文献类型：
--
作者：
HESS, G;ARNOLD, W;MEYER, KH
当用CsCl密度梯度离心分析时，通过蔗糖梯度反复沉淀，从先前与抗-HBc分离的Dane颗粒中释放出B核心抗原（HBcAg），分离成3个HBcAg群体。重HBcAg颗粒以1.355 gm/ml的密度成带，中等HBcAg颗粒以1.33 gm/ml的密度成带，轻HBcAg颗粒以1.30 gm/ml的密度成带。与重HBcAg颗粒一样，中间HBcAg颗粒含有DNA聚合酶活性，但HBcAg与DNA聚合酶活性的比值在两个人群中存在显著差异。用速率沉降离心法不能将中间HBcAg颗粒与重HBcAg颗粒分离。病毒DNA的大小及其单链缺口的大小在重和中等HBcAg群体中没有显著差异。中间HBcAg颗粒与重HBcAg颗粒在HBcAg多肽的量和所显示的HBcAg决定簇的数量上明显不同。
Hepatitis B core antigen (HBcAg) was released from Dane particles previously separated from anti-HBc by repeated pelleting through sucrose gradients separated into 3 HBcAg populations when analyzed by CsCl density gradient centrifugation. Heavy HBcAg particles banded at a density of 1.355 gm/ml, intermediate HBcAg particles at 1.33 gm/ml and light HBcAg particles at 1.30 gm/ml. Like heavy HBcAg particles, intermediate HBcAg particles contained DNA polymerase activity, but the ratio of HBcAg to DNA polymerase activity was significantly different in both populations. Intermediate HBcAg particles could not be separated from heavy HBcAg particles by rate sedimentation centrifugation. The size of the viral DNA and the size of its single-stranded gaps were not significantly different in heavy and intermediate HBcAg populations. The intermediate HBcAg particle evidently differs from the heavy HBcAg particle by the amount of HBcAg polypeptides and the number of HBcAg determinants exhibited.