Conditional gene knockout using Cre recombinase
Conditional gene knockout using Cre recombinase
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DOI：
10.1385/mb:17:3:269
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发表时间：
2001-03-01
影响因子：
2.6
通讯作者：
Sauer, B
中科院分区：
文献类型：
--
作者：
Le, YZ;Sauer, B
Cre重组酶已成为实现小鼠精准基因操作的重要工具。许多这些所需的遗传操作都依赖于 Cre 的能力，以指导在空间和时间上指定切除预先指定的 DNA 序列，该序列的两侧是 loxP 重组位点的直接重复副本。在小鼠中成功实现这种条件诱变取决于条件等位基因的仔细设计和 c rr 基因表达的可靠检测。这些程序包括 PCR、免疫组织化学和使用重组高效 GFP 标记的 Cre 蛋白。
Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional alleles and on reliable detection of c rr gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged Cre protein.