Long-term in vivo biomechanical properties and biocompatibility of poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) nerve conduits
Long-term in vivo biomechanical properties and biocompatibility of poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) nerve conduits
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DOI：
10.1016/j.biomaterials.2004.05.031
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Midha, R
中科院分区：
文献类型：
--
作者：
Belkas, JS;Munro, CA;Midha, R
人工移植物是用于周围神经修复的神经移植物的有前途的替代品，因为它们消除了与自体移植相关的并发症和缺点，例如供体部位发病率和组织可用性有限。我们合成了聚（甲基丙烯酸2-羟乙酯-甲基丙烯酸甲酯）（PHEMA-MMA）多孔管并研究了其体内功效。具体来说，我们研究了用于修复大鼠坐骨神经中手术产生的 10mm 神经间隙的 12 毫米长管的短期和长期稳定性和生物相容性。在植入之前，使用微机械测试仪对管进行体外分析，以测量施加负载时实现的位移。这些结果作为校准曲线，y = 6.8105 x -0.0073 (R-2 = 0.9750,n = 28)。用于 Nivo 吗啡管压缩测量。在体内，大多数 PHEMA-MMA 导管在长达 S 周的时间内保持其结构完整性。但其中 29% (4/14) 在 16 周内崩溃。有趣的是，塌陷的16周试管的管壁面积明显低于专利试管。试管基本上具有生物相容性；然而，一小部分 16 周管显示出慢性炎症的迹象，其特征是“手指状”组织延伸侵入内管方面、炎症细胞（其中一些被巨噬细胞 EDI 分裂）和巨细胞。管子也显示出钙化的迹象。前 8 至 16 周增加。为了克服这些问题，未来的神经导管将被重新设计，使其更加坚固且具有生物相容性。(C) 2004 Elsevier Ltd. 保留所有权利。
Artificial grafts are promising alternatives to nerve grafts for peripheral nerve repair because they obviate the complications and disadvantages associated with autografting such as donor site morbiditv and limited tissue availability. We have synthesized poly (2-hydroxyethyl methacrylate-co-methyl methacrylate) (PHEMA-MMA) porous tubes and studied their efficacy in vivo. Specifically, we studied the short- and long-term stability and biocompatibility of 12 mm long tubes for the repair of surgically created 10mm nerve gaps in rat sciatic nerves.Prior to implantation, tubes were analyzed in vitro using a micro-mechanical tester to measure displacement achieved with load applied. These results served as a calibration curve, y = 6.8105 x -0.0073 (R-2 = 0.9750,n = 28). for in Nivo morphonneiric tube compression measurements.In vivo, most of the PHEMA-MMA conduits maintained their structural integrity up to S weeks. but 29% (4/14) of them collapsed by 16 weeks. Interestingly, the tube wall area of collapsed 16-week tubes was significantly lower than those of patent tubes.Tubes were largely biocompatible; however, a small subset of 16-week tubes displayed signs of chronic inflammation characterized by "finger-like" tissue extensions invading the inner tube aspect, inflammatory cells (some of which were EDI divided by macrophages) and giant cells. Tubes also demonstrated signs of calcification. which increased front 8 to 16 weeks. To overcome these issue", future nerve conduits will be re-designed to be more robust and biocompatible. (C) 2004 Elsevier Ltd. All rights reserved.