Selective cell attachment to a biomimetic polymer surface through the recognition of cell-surface tags
Selective cell attachment to a biomimetic polymer surface through the recognition of cell-surface tags
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DOI：
10.1021/bc049707r
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发表时间：
2005-05-01
影响因子：
4.7
通讯作者：
Akiyoshi, K
中科院分区：
文献类型：
--
作者：
Iwasaki, Y;Tabata, E;Akiyoshi, K
合成了可以识别生物合成细胞表面标签的反应性磷酸胆碱聚合物来控制细胞附着。通过用 N-乙酰丙酰甘露糖胺 (ManLev) 处理，收获具有非天然碳水化合物作为细胞表面标签的人早幼粒细胞白血病细胞 (HL-60)。研究了 ManLev 处理的 HL-60 细胞与带有酰肼基团的 2-甲基丙烯酰氧基乙基磷酸胆碱 (MPC) 聚合物的附着情况。 HL-60 细胞是非粘附性的，未经 ManLev 处理不会粘附到任何聚合物表面。相比之下，ManLev处理的HL-60细胞在孵育15分钟后附着在聚[MPC-co-甲基丙烯酸正丁酯（BMA）-co-甲基丙烯酰肼（MH）]（PMBH）表面。附着在 PMBH 表面的细胞在孵育 24 小时后仍保持其天然形态和活力。另一方面，大约一半附着在聚（BMA-co-MH）（PBH）表面的HL-60细胞死亡。这些结果表明，聚合物中的 MH 单元充当细胞附着的锚，而 MPC 单元有助于保持聚合物表面上的细胞活力。在聚合物表面上进行 ManLev 处理的 HL-60 和荧光染色的人宫颈癌细胞 (HeLa) 的共培养。 ManLev 处理的 HL-60 细胞特异性附着于 PMBH 表面。相反，在 PBH 表面观察到 HL-60 和 HeLa 细胞。细胞与合成聚合物相互作用的控制可能有助于细胞集成生物传感器和生物医学设备的未来发展。
Reactive phosphorylcholine polymers, which can recognize biosynthetic cell-surface tags, were synthesized to control cell attachment. Human promyelocytic leukemia cells (HL-60) with unnatural carbohydrates as cell-surface tags were harvested by treatment with N-levulinoylmannosamine (ManLev). The attachment of ManLev-treated HL-60 cells to 2-methacryloyloxyethyl phosphory1choline (MPC) polymers with hydrazide groups was studied. HL-60 cells, which are nonadhesive, did not attach to any polymer surface without ManLev treatment. In contrast, ManLev-treated HL-60 cells attached to a poly [MPC-co-n-butyl methacrylate (BMA)-co-methacryloyl hydrazide (MH)] (PMBH) surface following 15 min of incubation. The cells that attached to the PMBH surface retained their native morphology and viability for 24 h of incubation. On the other hand, approximately half of the HL-60 cells that attached to the poly(BMA-co-MH) (PBH) surface died. These results suggest that MH units in the polymer act as anchors for cell attachment and MPC units help to preserve cell viability on a polymer surface. The coculture of ManLev-treated HL-60 and fluorescence-stained human uterine cervical cancer cells (HeLa) was carried out on polymer surfaces. ManLev-treated HL-60 cells specifically attached to the PMBH surface. In contrast, both HL-60 and HeLa cells were observed on the PBH surface. The control of cellular interactions with synthetic polymers may be useful for the future development of cell-integrated biosensors and biomedical devices.