Evidence for essential histidine and cysteine residues in calcium/calmodulin-sensitive cyclic nucleotide phosphodiesterase.
Evidence for essential histidine and cysteine residues in calcium/calmodulin-sensitive cyclic nucleotide phosphodiesterase.
复制标题
钙/钙调蛋白敏感的环核苷酸磷酸二酯酶中必需的组氨酸和半胱氨酸残基的证据。
DOI：
10.1021/bi00241a018
复制
发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Wells,JN
中科院分区：
文献类型：
--
作者：
Ahn,HS;Foster,M;Foster,C;Sybertz,E;Wells,JN
材料与方法DEP、[，4C]DEP(9.4mCI/mmol)、NEM、DTNB、NH-OH、DTT、BR-cGMP、Mix、cGMP、L组氨酸、蛇毒等大多数试剂级化合物均购自Sigma Chemical Co.液体鸡尾酒是来自Beckman的，准备好的安全和准备好的蛋白质。DEAE-Sephacel和CaM-Affigel15分别从微孔和Bio-Rad中获得。DEP解决方案
Materials and MethodsDEP,[, 4C] DEP (9.4 mCi/mmol), NEM, DTNB, NH2OH, DTT, Br-cGMP, MIX, cGMP, L-histidine, Crotaius atrox (snake venom), and most other chemicals, all of reagent grade, were obtained from Sigma Chemical Co.[3H] cGMP (5.7 Ci/mmol) was purchased from DuPont NEN. Liquid scin-tillation cocktails, Ready Safe and Ready Protein, were from Beckman. DEAE-Sephacel and CaM-Affigel 15 were obtained from Millipore and Bio-Rad, respectively. DEP solution