Supplement zinc as an effective treatment for spinal cord ischemia/reperfusion injury in rats
Supplement zinc as an effective treatment for spinal cord ischemia/reperfusion injury in rats
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DOI：
10.1016/j.brainres.2013.12.015
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发表时间：
2014-01-30
期刊：
影响因子：
2.9
通讯作者：
Mei, Xifan
中科院分区：
文献类型：
--
作者：
Wang, Yansong;Su, Ribao;Mei, Xifan
目的：脑源性神经营养因子（BDNF）在脊髓损伤（SCI）的病理生理过程和治疗中起着重要作用。因此，锌调节BDNF及其受体在中枢神经系统中的表达，其机制尚不清楚。本研究旨在探讨补锌是否能减轻大鼠脊髓缺血再灌注（I/R）损伤的神经功能损害以及锌转运蛋白1（ZnT-1）的作用。方法：100只雄性SD大鼠随机分为4组。缺锌饮食模型组（ZD）、锌充足饮食模型组（ZA）和高锌饮食模型组（ZH）大鼠自由进食含锌5、30和180 mg/kg的纯净饲料。假手术组大鼠在不夹闭主动脉的情况下进行剖腹手术，并以ZA饮食（30 mg Zn/kg）喂养。神经功能按Tarlov评分进行评分。取脊髓（L5）节段进行组织学检查、自组织金相（AMG）分析、髓过氧化物酶（MPO）活性分析、ZnT-1和BDNF的表达。结果如下：ZH组大鼠在4个观察时间点的神经功能评分均高于ZD组和ZA组（p < 0.05），组织学改变轻于ZD组和ZA组（p < 0.05），MPO活性降低（p < 0.05）。
Objective: Brain-derived neurotrophic factor (BDNF) plays a key role in the pathophysiology process and therapy of spinal cord injury (SCI). Accordingly, zinc regulates the expression of BDNF and its receptor in the central nervous system, the mechanism of which is still unknown. The present study investigates whether supplement zinc could reduce neurological damage in a rat model, with spinal cord ischemia-reperfusion (I/R) injury and how the effect of zinc transporter 1(ZnT-1) was involved. Methods: 100 Sprague-Dawley male rats were randomly and evenly divided into four groups. They were subjected to spinal cord ischemia by clamping the abdominal aorta for 45 min. Rats in the zinc-deficient dietary model group (ZD), zinc-adequate dietary model group (ZA), and zinc-high dietary model group (ZH) were given free access to purified diet, containing 5, 30, or 180 mg Zn/kg. Sham operation rats were subjected to laparotomy without clamping of the aorta and were fed by ZA diet (30 mg Zn/kg). Neurological function was scored by Tarlov's score. The spinal cord segments (L5) were harvested for histological examination, auto-metallographic (AMG) analysis, myeloperoxidase (MPO) activity analysis, expression of ZnT-1 and BDNF. Results: The rats in the ZH group have shown the higher neurological scores, slighter histological changes and the attenuated MPO activity, compared with those in the ZD and ZA groups at the four observation time points (p