Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy — shaking and extra-light supplementation approach
Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy — shaking and extra-light supplementation approach
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DOI：
10.1016/j.ijhydene.2009.10.020
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发表时间：
2009-12
影响因子：
7.2
通讯作者：
Xu Li;Yonghong Wang;Siliang Zhang;J. Chu;Z. Ming;Mingzhi Huang;Y. Zhuang
中科院分区：
文献类型：
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作者：
Xu Li;Yonghong Wang;Siliang Zhang;J. Chu;Z. Ming;Mingzhi Huang;Y. Zhuang
生物氢因其作为传统制氢方法的可持续替代品的潜力而受到关注。本研究在38-ml的RCVBN培养基厌氧光生物反应器中，研究了光照强度和培养方式（静置和振荡培养）对球形红杆菌ZX-5细胞生长和产氢的影响。因此，本研究以苹果酸盐为唯一碳源，建立了一种新的振荡和超光补充（SELS）方法来提高R. sphaeroides ZX-5的光养产氢能力。菌株ZX-5振荡培养的最佳光照条件提高到7000 ~ 8000lux，显著高于静培养（4000 ~ 5000lux）。在震动和高光照（7000 ~ 8000lux）条件下，培养物能有效促进光- h2production，与静置和4000 ~ 5000lux条件下的培养相比，最大产氢率和平均产氢率分别提高了59%和56%。采用SELS法产氢率最高，为165.9ml H2/lh。据我们所知，这是目前非固定化紫色无硫（PNS）细菌产氢率最高的记录。利用SELS方法实现光产氢的最佳性能是提高光养产氢效率的可持续和经济可行策略的良好选择。
Biohydrogen has gained attention due to its potential as a sustainable alternative to conventional methods for hydrogen production. In this study, the effect of light intensity as well as cultivation method (standing- and shaking-culture) on the cell growth and hydrogen production of Rhodobacter sphaeroides ZX-5 were investigated in 38-ml anaerobic photobioreactor with RCVBN medium. Thus, a novel shaking and extra-light supplementation (SELS) approach was developed to enhance the phototrophic H2production by R. sphaeroides ZX-5 using malate as the sole carbon source. The optimum illumination condition for shaking-culture by strain ZX-5 increased to 7000–8000lux, markedly higher than that for standing-culture (4000–5000lux). Under shaking and elevated illumination (7000–8000lux), the culture was effective in promoting photo-H2production, resulting in a 59% and 56% increase of the maximum and average hydrogen production rate, respectively, in comparison with the culture under standing and 4000–5000lux conditions. The highest hydrogen-producing rate of 165.9ml H2/lh was observed under the application of SELS approach. To our knowledge, this record is currently the highest hydrogen production rate of non-immobilized purple non-sulphur (PNS) bacteria. This optimal performance of photo-H2production using SELS approach is a favorable choice of sustainable and economically feasible strategy to improve phototrophic H2production efficiency.