Tocotrienols from palm oil as effective inhibitors of protein oxidation and Lipid peroxidation in rat liver microsomes
Tocotrienols from palm oil as effective inhibitors of protein oxidation and Lipid peroxidation in rat liver microsomes
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DOI：
10.1023/a:1006853419214
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发表时间：
1997-05-01
影响因子：
4.3
通讯作者：
Basiron, Y
中科院分区：
文献类型：
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作者：
Kamat, JP;Sarma, HD;Basiron, Y
棕榈油中的生育三烯酚在大鼠肝微粒体中表现出显着的抑制抗坏血酸-Fe2+ 和光敏作用诱导的氧化损伤的能力，涉及不同的机制。棕榈油中富含生育三烯酚的馏分 (TRF) 被尝试作为 α-生育酚的更经济、更有效的替代品，显示出对蛋白质氧化和脂质过氧化的时间和浓度依赖性抑制。它对蛋白质氧化更有效。 TRF 的抑制程度随不同的过氧化产物（例如共轭二烯、脂质氢过氧化物和硫代巴比妥酸反应物质（TBARS））而变化。在 TRF 的成分中，γ-生育三烯酚最有效，其次是其 α 和 δ 异构体。一般来说，在 5 μM 的低浓度下，TRF 能够显着程度地防止氧化损伤（孵育 1 小时时抑制 37% 的蛋白质氧化和 27-30% 的脂质过氧化）。 TRF的保护能力（5μM时为30.1%，形成TBARS）显着高于维生素E的主要形式α-生育酚（相同条件下为16.5%）。因此，我们的研究表明，棕榈油的这种成分可以被认为是一种有效的天然抗氧化剂补充剂，能够保护细胞膜免受氧化损伤。
Tocotrienols from palm oil showed significant ability to inhibit oxidative damage induced by ascorbate-Fe2+ and photosensitization, involving different mechanisms, in rat liver microsomes. The tocotrienol-rich fraction from palm oil (TRF), being tried as a more economical and efficient substitute for alpha-tocopherol, showed time- and concentration-dependent inhibition of protein oxidation as well as lipid peroxidation. It was more effective against protein oxidation. The extent of inhibition by TRF varied with different peroxidation products such as conjugated dienes, lipid hydroperoxides and thiobarbituric acid reactive substances (TBARS). Among the constituents of TRF, gamma-tocotrienol was the most effective followed by its alpha- and delta-isomers. In general, at a low concentration of 5 mu M, TRF was able to prevent oxidative damage to significant extent (37% inhibition of protein oxidation and 27-30% of lipid peroxidation at 1 h of incubation). The protective ability of TRF (30.1% at 5 mu M with TBARS formation) was significantly higher than that of the dominant form of vitamin E, alpha-tocopherol (16.5% under same conditions). Hence our studies indicate that this fraction from palm oil can be considered as an effective natural antioxidant supplement capable of protecting cellular membranes against oxidative damage.