A conjugated carbon-dot-tyrosinase bioprobe for highly selective and sensitive detection of dopamine
A conjugated carbon-dot-tyrosinase bioprobe for highly selective and sensitive detection of dopamine
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DOI：
10.1039/c8an01659c
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发表时间：
2019-01-21
期刊：
影响因子：
4.2
通讯作者：
Lin, Hengwei
中科院分区：
文献类型：
--
作者：
Tang, Zhongdi;Jiang, Kai;Lin, Hengwei
本工作通过荧光碳点（CD）与酪氨酸酶（TYR）的共价键合，设计并制备了一种检测多巴胺的生物探针。该生物探针（CDs-TYR）可催化多巴胺氧化生成多巴醌，并通过电子转移机制使其荧光猝灭。CD的荧光强度呈多巴胺浓度依赖性下降，这为多巴胺定量奠定了基础。生物探针提供了一个广泛的线性范围从0.1至6.0 μ M的多巴胺传感。此外，由于共轭TYR的特异性催化特性，生物探针对多巴胺具有优异的选择性。此外，生物探针被成功地用于检测多巴胺的人血清中添加。据我们所知，这是通过缀合CD和酶构建生物探针的第一个例子。这项工作将开辟新的机会，开发基于CD的光诱导电子转移生物探针的其他分析物通过连接典型的酶到CD。
In this work, a bioprobe for the detection of dopamine was designed and fabricated through covalently linking fluorescent carbon dots (CDs) and tyrosinase (TYR). The bioprobe (named CDs-TYR) can catalyze oxidation of dopamine and produce dopaquinone, and consequently the fluorescence of the CDs was quenched due to an efficient electron transfer mechanism from excited CDs to dopaquinone. The fluorescence intensity of CDs decreased in a dopamine-concentration-dependent manner, which built the foundation of dopamine quantification. The bioprobe provided a wide linear range from 0.1 to 6.0 mu M for dopamine sensing. Additionally, excellent selectivity of the bioprobe to dopamine was achieved because of the specific catalytic character of the conjugated TYR. Furthermore, the bioprobe was successfully employed for the detection of dopamine in spiked human serum. To the best of our knowledge, this is the first example of the construction of a bioprobe through conjugating CDs and an enzyme. This work would open new opportunities to develop CD-based photoinduced electron transfer bioprobes for other analytes via linking typical enzymes onto CDs.