FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis
FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis
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DOI：
10.1038/s41467-019-12379-z
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发表时间：
2019
影响因子：
16.6
通讯作者：
Mo Beixin
中科院分区：
文献类型：
--
作者：
You Chenjiang;He Wenrong;Hang Runlai;Zhang Cuiju;Cao Xiaofeng;Guo Hongwei;Chen Xuemei;Cui Jie;Mo Beixin
Plant microRNAs (miRNAs) associate with ARGONAUTE1 (AGO1) to direct post- transcriptional gene silencing and regulate numerous biological processes. Although AGO1 predominantly binds miRNAs in vivo, it also associates with endogenous small interfering RNAs (siRNAs). It is unclear whether the miRNA/siRNA balance affects miRNA activities. Here we report that FIERY1 (FRY1), which is involved in 5′−3′ RNA degradation, regulates miRNA abundance and function by suppressing the biogenesis of ribosomal RNA-derived siRNAs (risiRNAs). In mutants of FRY1 and the nuclear 5′−3′ exonuclease genes XRN2 and XRN3, we find that a large number of 21-nt risiRNAs are generated through an endogenous siRNA biogenesis pathway. The production of risiRNAs correlates with pre-rRNA processing defects in these mutants. We also show that these risiRNAs are loaded into AGO1, causing reduced loading of miRNAs. This study reveals a previously unknown link between rRNA processing and miRNA accumulation.
