The cellular proteome is affected by a gelsolin (BbGEL1) during morphological transitions in aerobic surface versus liquid growth in the entomopathogenic fungus Beauveria bassiana
The cellular proteome is affected by a gelsolin (BbGEL1) during morphological transitions in aerobic surface versus liquid growth in the entomopathogenic fungus Beauveria bassiana
复制标题
昆虫病原真菌白僵菌在有氧表面与液体生长的形态转变过程中，细胞蛋白质组受到凝溶胶蛋白 (BbGEL1) 的影响
DOI：
10.1111/1462-2920.13500
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发表时间：
2016-11-01
影响因子：
5.1
通讯作者：
Ying, Sheng-Hua
中科院分区：
文献类型：
--
作者：
He, Pu-Hong;Dong, Wei-Xia;Ying, Sheng-Hua
凝溶胶蛋白超家族包括七个蛋白质成员：凝溶胶蛋白、绒毛蛋白、adseverin、CapG、advillin、supervillin和flightless I。凝溶胶蛋白是一类含有三个或六个凝溶胶蛋白样结构域的肌动蛋白结合蛋白，在真核生物中对肌动蛋白动力学和细胞过程的重塑起着重要作用。昆虫病原真菌球孢白僵菌表达一种独特的CapG蛋白（BbGEL 1），该蛋白含有三个凝溶胶样结构域。BbGEL1p在菌丝生长过程中与肌动蛋白相关，并在有氧和淹没条件下的真菌形态转变中起着重要作用。Delta BbGEL 1突变体显示出异常的产孢子结构，其分别使分生孢子和芽生孢子产量降低约70%和90%。如局部和血腔内注射试验所示，Delta BbGEL 1突变体的毒力显著降低。两个比较蛋白质组学分析表明，BbGEL 1在分生孢子和芽生孢子发育过程中具有显著不同的作用，揭示了BbGEL 1在相应发育过程中的潜在作用靶点。此外，作为BbGEL 1的重叠下游蛋白，疏水蛋白样蛋白基因BbHyd3是分生孢子形成所需的，但在芽生孢子形成中具有负面作用。我们的研究结果表明，除了作为一个肌动蛋白相互作用蛋白的功能，BbGEL 1有助于真菌形态转变通过广泛的遗传途径。
The gelsolin superfamily includes seven protein members: gelsolin, villin, adseverin, CapG, advillin, supervillin and flightless I. The gelsolin proteins are actin-binding proteins that contain three or six gelsolin-like domains, and they play important roles in remodelling actin dynamics and cellular processes in eukaryotes. The entomopathogenic fungus Beauveria bassiana expresses a unique CapG protein (BbGEL1) that contains three gelsolin-like domains. BbGEL1p is associated with actin during mycelial growth and plays an important role in fungal morphological transitions under both aerobic and submerged conditions. The Delta BbGEL1 mutant displays abnormal spore-producing structures that reduce the conidial and blastospore yields by approximately 70% and 90% respectively. The virulence of the Delta BbGEL1 mutant is notably reduced as indicated by topical and intrahemocoel injection assays. Two comparative proteomics analyses indicated that BbGEL1 has significantly different roles in the development of conidia and blastospores, and the results revealed the potential targets of BbGEL1 in the corresponding developmental processes. Additionally, as an overlapping downstream protein of BbGEL1, the hydrophobin-like protein gene BbHyd3 is required for conidiation but has a negative role in blastospore formation. Our findings indicate that in addition to its function as an actin-interacting protein, BbGEL1 contributes to fungal morphological transitions via broad genetic pathways.