Nucleotide polymorphism at the xanthine dehydrogenase locus in Drosophila pseudoobscura.
Nucleotide polymorphism at the xanthine dehydrogenase locus in Drosophila pseudoobscura.
复制标题
果蝇黄嘌呤脱氢酶位点的核苷酸多态性。
DOI：
10.1093/oxfordjournals.molbev.a040708
复制
发表时间：
1992
影响因子：
10.7
通讯作者：
Veuille,M
中科院分区：
文献类型：
--
作者：
Riley,MA;Kaplan,SR;Veuille,M
用聚丙烯酰胺连续电泳法研究了拟暗果蝇（Drosophila pseudobscura）黄嘌呤脱氢酶（XDH）的20种等位酶。DNA序列测定的7个菌株的Xdh基因座，代表六个等位酶类在这里。在检测的5，456个位点中，180个是多态性的，其中27个多态性发生在非同义或替代位点。XDH等位酶类之间平均有9个氨基酸不同，其中85%的多态性氨基酸单独代表。在Xdh观察到的变异水平和模式表明，该物种的有效种群规模相当大，即，数量级为2 × 10（6）--而且取样的种群相当古老。此外，通过两个统计检验判断，在Xdh观察到的核苷酸多态性水平与分子进化中性理论的预测是一致的。
Sequential polyacrylamide electrophoresis has revealed 20 allozymes of xanthine dehydrogenase (XDH) in Drosophila pseudoobscura. DNA sequence determination of seven isolates of the Xdh locus that represent six allozyme classes are presented here. Of the 5,456 sites examined, 180 are polymorphic, with 27 polymorphisms occurring at nonsynonymous, or replacement, sites. An average of nine amino acids differ between XDH allozyme classes, with 85% of the polymorphic amino acids singly represented. The level and pattern of variation observed at Xdh argue that the effective population size of the species is quite large--i.e., on the order of 2 x 10(6)--and that the populations sampled are quite ancient. In addition, as judged by two statistical tests, the levels of nucleotide polymorphism observed at Xdh are compatible with predictions from the neutral theory of molecular evolution.