Evaluation of the role of mitotic instability in karyotypically normal men with oligozoospermia
Evaluation of the role of mitotic instability in karyotypically normal men with oligozoospermia
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DOI：
10.1016/s0015-0282(99)00467-7
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发表时间：
2000-01-01
影响因子：
6.7
通讯作者：
Lewis-Jones, DI
中科院分区：
文献类型：
--
作者：
Gazvani, MR;Wilson, EDA;Lewis-Jones, DI
目的：估计核型正常的不育男性和生育供体的体细胞中性染色体非整倍体的发生率。设计：前瞻性、两阶段、对照研究。设置：教学医院的生殖医学和医学遗传学单位。患者：10名少精子症患者和10名经证实具有生育能力的精子供体，均具有正常核型46 XY。干预措施：外周血淋巴细胞的多色荧光原位杂交（FISH）使用含有X着丝粒的α卫星DXZ 1和Y长臂异染色质区的DYZ 1以及染色体的放射性标记的α卫星D18 Z1的探针混合物。主要结果测量：两组性染色体非整倍体的发生率。结果：与供精者相比，不明原因少精子症不育男性的性染色体非整倍体增加了13倍结论：不明原因偏精子症患者存在固有的有丝分裂不稳定性。（Fertil Steril（R）2000;73：51-5. (C)1999年由美国生殖医学协会。
Objective: To estimate the incidence of sex chromosome aneuploidies in the somatic cells of karyotypically normal infertile men and fertile donors.Design: A prospective, two-phase, controlled study.Setting: Reproductive medicine and medical genetics units of a teaching hospital.Patient(s): Ten patients with oligozoospermia and 10 sperm donors with proved fertility, all with a normal karyotype 46 XY.Intervention(s): Multicolor fluorescence in situ hybridization (FISH) of peripheral blood lymphocytes using a probe cocktail containing the alpha satellites DXZ1 for the X centromere and DYZ1 for the heterochromatic region of the long arm of the Y and the radiolabeled alpha satellite D18Z1 for chromosome is.Main Outcome Measure(s): The incidence of sex chromosome aneuploidies in both groups.Result(s): A 13-fold increase in sex chromosome aneuploidies was observed in the somatic cells of infertile men with "unexplained" oligozoospermia compared to donors (P=.008).Conclusion(s): These findings provide suggestive evidence for the existence of an inherent mitotic instability in men with unexplained obligozoospermia. (Fertil Steril(R) 2000;73:51-5. (C) 1999 by American Society for Reproductive Medicine.)