Effect of genotype on steady-state CYP1A1 gene expression in human peripheral lymphocytes.
Effect of genotype on steady-state CYP1A1 gene expression in human peripheral lymphocytes.
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DOI：
10.1016/s0006-2952(02)01546-0
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发表时间：
2003-02
影响因子：
5.8
通讯作者：
S. Garte;S. Ganguly;E. Taioli
中科院分区：
文献类型：
--
作者：
S. Garte;S. Ganguly;E. Taioli
We have analyzed the steady-state levels of cytochrome P-450 1A1 (CYP1A1) mRNA in peripheral blood lymphocytes of 177 individuals with various CYP1A1 genotypes using a quantitative reverse transcriptase–polymerase chain reaction technique that makes use of a homologous internal standard for accurate quantitation. We found no effects of ethnicity, age, or smoking status on CYP1A1 gene expression in this population. We did see a significant 2-fold increase in the mean level of CYP1A1 mRNA in women compared with men for both Caucasians and African Americans. We observed no effect of the African American-specific polymorphism (CYP1A1∗3) on expression of the gene. However, we found a significant 3-fold decrease in expression associated with the homozygous MspI restriction fragment length polymorphism (CYP1A1∗2A/∗2A).
