Physicochemical characterization and biological effects of inulin enzymatically synthesized from sucrose.
Physicochemical characterization and biological effects of inulin enzymatically synthesized from sucrose.
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DOI：
10.1021/jf048711u
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发表时间：
2005-01
影响因子：
6.1
通讯作者：
T. Wada;J. Sugatani;E. Terada;M. Ohguchi;M. Miwa
中科院分区：
文献类型：
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作者：
T. Wada;J. Sugatani;E. Terada;M. Ohguchi;M. Miwa
我们首先开发了使用来自芽孢杆菌属217 C-1的酶从蔗糖生产菊粉的方法。合成菊粉由具有d-果糖与一个末端葡萄糖的β（2-1）键的线性聚合物组成。合成的菊粉具有与植物来源的菊粉相似的性质（pH和热稳定性、美拉德反应和体外发酵）。显著的区别是菊糖链长的多分散性。合成的菊糖果糖聚合度范围较窄，在水中的溶解性比植物来源的菊糖好。合成菊粉（5%，w/w）治疗12周，降低了高脂肪和高蔗糖补充饮食大鼠的体重和血清及肝脏脂质的升高，以及标准饮食大鼠的血糖升高。合成的菊粉（15%，w/w）显着抑制糊精负载后的人类健康受试者的血糖升高。这些结果表明，每日摄入的合成菊粉调节碳水化合物和脂质代谢。
We first developed the method to produce inulin from sucrose using an enzyme from Bacillus sp. 217C-1. Synthesized inulin consists of a linear polymer having beta(2-1) linkages of d-fructose with one terminal glucose. The synthesized inulin has similar properties (pH and thermal stability, Maillard reaction, and in vitro fermentation) to plant-derived inulin. The marked difference is the polydispersity of the inulin chain length. Synthesized inulin with a narrow degree range of fructose polymerization shows better solubility in water than plant-derived inulin. Synthesized inulin (5%, w/w) treatment for 12 weeks reduced the elevation in body weight and serum and liver lipids in rats fed high fat- and high sucrose-supplemented diets, and blood glucose in rats fed a standard diet. Synthesized inulin (15%, w/w) significantly suppressed the elevation in blood glucose of human healthy subjects after dextrin loading. These results suggest that daily intake of synthesized inulin modulates carbohydrate and lipid metabolism.