CHARACTERIZATION OF FIBROBLASTS DERIVED FROM HUMAN PERIODONTAL-LIGAMENT AND GINGIVA
CHARACTERIZATION OF FIBROBLASTS DERIVED FROM HUMAN PERIODONTAL-LIGAMENT AND GINGIVA
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DOI：
10.1902/jop.1990.61.2.103
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发表时间：
1990-02-01
影响因子：
4.3
通讯作者：
COCHRAN, DL
中科院分区：
文献类型：
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作者：
MARIOTTI, A;COCHRAN, DL
在细胞培养中比较了源自人牙周膜（PDLF）和牙龈（GF）的成纤维细胞的生长特性和大分子合成。从外植体中分离细胞，并以 500,000 个细胞/100 mm 培养皿（第 0 天）铺板，每天更换培养基。通过流式微荧光分析获得 DNA 直方图，以确认细胞培养物的生长状态。根据细胞数量和细胞周期分析确定，人 PDLF 培养物在第 6 天融合，而 GF 在第 4 天融合。最初，GF 培养物中对数生长细胞的 DNA 含量显着更高；然而，当PDLF 培养物汇合时，DNA 含量和细胞数量更高。直到第 7 天，GF 中的总蛋白含量略高于 PDLF，但这种差异并不显着。对胶原蛋白和非胶原蛋白合成的分析显示，与 PDLF 培养物相比，GF 培养物中非胶原蛋白合成的趋势更大。对 GF 和 PDLF 培养基中糖胺聚糖的分析揭示了相似的成分分布。在细胞部分中，GF 比 PDLF 培养物含有更多量的透明质酸和肝素，以及更少量的硫酸软骨素 A 和 C。结果表明，PDLF和GF的生长特性虽然在许多方面相似，但在增殖速率和大分子合成方面确实表现出特定的差异。在这些参数中观察到的差异在体内事件期间可能很重要，例如引导组织再生，其中在牙龈结缔组织和牙周膜结缔组织之间观察到显着的功能差异。
Growth characteristics and macromolecular synthesis of fibroblasts derived from human periodontal ligment (PDLF) and gingiva (GF) have been compared in cell culture. Cells were isolated from explants and plated at 500,000 cells/100 mm culture dish (day 0) with daily changes of culture medium. DNA histograms were obtained by flow microfluorimetric analysis to confirm the growth state of the cell cultures. Human PDLF cultures became confluent at day 6 as determined by cell number and cell cycle analysis while GF were confluent by day 4. Initially, DNA content of logarithmically growing cells was significantly greater in GF cultures; however, when confluent, DNA content and cell number was greater in PDLF cultures. Total protein content in GF was slightly greater than PDLF until day 7 but this difference was not significant. Analysis of collagen and noncollagen protein synthesis revealed a greater trend in noncollagen protein synthesis in the GF cultures compared to PDLF cultures. Analysis of glycosaminoglycans in the culture medium of GF and PDLF revealed similar distribution of components. In the cellular fraction, GF had greater amounts of hyaluronic acid and heparin and lesser amounts of chondroitin sulfates A and C than PDLF cultures. The results indicate that the growth characteristics of PDLF and GF, although similar in many respects, do exhibit specific differences in proliferative rates and macromolecular synthesis. The differences observed in these parameters may be important during in vivo events, such as guided tissue regeneration, where significant functional differences are observed between gingival connective tissue and periodontal ligament connective tissue.