Activity-Based Probe for Screening of High-Colibactin Producers from Clinical Samples
Activity-Based Probe for Screening of High-Colibactin Producers from Clinical Samples
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DOI：
10.1021/acs.orglett.9b01345
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发表时间：
2019-06-21
期刊：
影响因子：
5.2
通讯作者：
Watanabe, Kenji
中科院分区：
文献类型：
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作者：
Hirayama, Yuichiro;Tsunematsu, Yuta;Watanabe, Kenji
虽然高产大肠杆菌素的大肠杆菌被认为与结直肠肿瘤的发生有关，但由于无法充分分离大肠杆菌素，这项研究是复杂的部分。在这里，我们创建了由大肠杆菌素成熟肽酶ClbP激活的荧光探针，以识别高产大肠杆菌素的菌株。我们的探针作为一个有价值的临床诊断工具，允许简单的高通量诊断筛选的临床样品。此外，该探针还允许鉴定高大肠杆菌素生产者，这将有助于推进我们对大肠杆菌素生物合成的理解。
While high-colibactin-producing Escherichia coil is thought to be associated with colorectal oncogenesis, this study is complicated part due to an inability to isolate colibactin adequately. Here, we created fluorescent probes activated by ClbP, the colibactin-maturing peptidase, to identify high-colibactin-producing strains. Our probe served as a valuable clinical diagnostic tool that allowed simple high throughput diagnostic screening of clinical samples. Furthermore, the probe also allowed identification of high-colibactin producers that would help advance our understanding of colibactin biosynthesis.