Soluble HLA-G1 triggers CD95/CD95 ligand-mediated apoptosis in activated CD8+ cells by interacting with CD8
Soluble HLA-G1 triggers CD95/CD95 ligand-mediated apoptosis in activated CD8+ cells by interacting with CD8
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DOI：
10.4049/jimmunol.164.12.6100
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发表时间：
2000-06-15
影响因子：
4.4
通讯作者：
Le Bouteiller, P
中科院分区：
文献类型：
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作者：
Fournel, S;Aguerre-Girr, M;Le Bouteiller, P
非多态的可溶性人类白细胞抗原-G1(sHLA-G1)亚型已被报道由母胎界面的滋养层细胞分泌，提示它可能在妊娠期间起免疫调节剂的作用。在本文中，我们报道了亲和纯化的β(2)-微球蛋白相关的sHLA-G1诱导活化的CD8(+)外周血细胞的凋亡，但不能诱导静止的CD8(+)外周血细胞的凋亡，我们通过Western blotting证明了sHLA-G1增强了活化的CD8+细胞的CD95配体的表达。CD95拮抗剂单抗(ZB4)或可溶性重组CD95-Fc可抑制细胞毒作用，表明细胞凋亡是通过Cn95/CD95配体途径介导的。最后，我们证明了这种由sHLA-G1诱导的细胞凋亡依赖于与CDS分子的相互作用，细胞死亡被各种CD8单抗阻断。
The nonpolymorphic soluble HLA-G1 (sHLA-G1) isoform has been reported to be secreted by trophoblast cells at the materno fetal interface, suggesting that it may act as immunomodulator during pregnancy. In this paper, we report that affinity-purified beta(2)-microglobulin-associated sHLA-G1 triggered apoptosis in activated, but not resting CD8(+) peripheral blood cells, We demonstrate by Western blotting that sHLA-G1 enhanced CD95 ligand expression in activated CD8+ cells. Cytotoxicity was inhibited by preincubation of the cells with a CD95 antagonist mAb (ZB4) or a soluble recombinant CD95-Fc, indicating that apoptosis is mediated through the Cn95/CD95 ligand pathway, Finally, we show that such sHLA-G1-induced apoptosis depends on the interaction with CDS molecules, with cell death being blocked by various CD8 mAbs.