Recent Advances of Activatable Molecular Probes Based on Semiconducting Polymer Nanoparticles in Sensing and Imaging.
Recent Advances of Activatable Molecular Probes Based on Semiconducting Polymer Nanoparticles in Sensing and Imaging.
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DOI：
10.1002/advs.201600481
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发表时间：
2017-06
期刊：
影响因子：
--
通讯作者：
Pu K
中科院分区：
文献类型：
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作者：
Lyu Y;Pu K
根据感兴趣的靶标改变信号的分子探针在基础生物学和医学中发挥着至关重要的作用。半导体聚合物纳米粒子（SPN）最近作为新一代纯有机光子纳米剂出现，具有生物应用所需的特性。特别是，SPN的可调谐光学特性使其能够开发成光致发光、化学发光和光声探针，其中SPN通常分别用作发光和光声探针的能量供体和内参。此外，简单的表面改性和颗粒内工程提供了多功能性，使它们能够响应各种生物学和病理学上重要的物质和指标，包括小分子介质、蛋白质、pH 和温度。本文重点介绍用于传感和成像的基于 SPN 的可激活分子探针的开发最新进展。详细讨论了这些探针的设计和应用，并分析了进一步将它们推进生命科学的当前挑战。
Molecular probes that change their signals in response to the target of interest have a critical role in fundamental biology and medicine. Semiconducting polymer nanoparticles (SPNs) have recently emerged as a new generation of purely organic photonic nanoagents with desirable properties for biological applications. In particular, tunable optical properties of SPNs allow them to be developed into photoluminescence, chemiluminescence, and photoacoustic probes, wherein SPNs usually serve as the energy donor and internal reference for luminescence and photoacoustic probes, respectively. Moreover, facile surface modification and intraparticle engineering provide the versatility to make them responsive to various biologically and pathologically important substances and indexes including small‐molecule mediators, proteins, pH and temperature. This article focuses on recent advances in the development of SPN‐based activatable molecular probes for sensing and imaging. The designs and applications of these probes are discussed in details, and the present challenges to further advance them into life science are also analyzed.