2 TIGHTLY LINKED SILENT CASSETTES IN THE MATING-TYPE REGION OF SCHIZOSACCHAROMYCES-POMBE
2 TIGHTLY LINKED SILENT CASSETTES IN THE MATING-TYPE REGION OF SCHIZOSACCHAROMYCES-POMBE
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DOI：
10.1007/bf00417816
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发表时间：
1984-01-01
期刊：
影响因子：
2.5
通讯作者：
EGEL, R
中科院分区：
文献类型：
--
作者：
EGEL, R
遗传学证据表明存在两个沉默的盒mat2-P和mat3-M，它们都位于S.pombe交配型区域可表达位点mat1的右侧。在交配类型的切换期间，MAT1处的驻留盒式磁带被来自1个静默座位的相反交配类型信息的副本所取代。通常情况下，开关在2个子细胞中的1个细胞中有效，从而允许有效的姐妹细胞结合。在Swi突变体中，早在孢子萌发后的第一次分裂就可以观察到交配型转换，尽管频率较低。从基因上讲，这两个无声磁带紧密相连，以至于在10-3厘米[厘米]的分辨率下，没有观察到mat2和mat3之间的交叉。
Genetic evidence is presented for the presence of 2 silent cassettes mat2-P and mat3-M, which both map to the right of the expressible site mat1 of the mating-type region in S. pombe. During a switch of mating type, the resident cassette at mat1 is replaced by a copy of opposite mating-type information from 1 of the silent loci. Usually the switch becomes effective in 1 of 2 daughter cells, thus allowing for efficient sister-cell conjugation. In swi mutants, mating-type switching can be observed as early as for the 1st division after spore germination, albeit at a lower frequency. Genetically the 2 silent cassettes are linked so tightly that no crossovers were observed between mat2 and mat3 at a resolution of 10-3 cM [centimorgan].