Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining
Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining
复制标题
通过基因组挖掘快速发现担子菌二萜合酶并进行功能表征
DOI：
10.1016/j.fgb.2019.03.007
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发表时间：
2019-07-01
影响因子：
3
通讯作者：
Gao, Wei
中科院分区：
文献类型：
--
作者：
Li, Xin-Lin;Xu, Yu-Xing;Gao, Wei
担子菌类真菌是具有生物活性的二萜类次级代谢产物的丰富来源。然而，与在植物和子囊菌中鉴定的大量二萜类糖苷酶（di-TPS）相比，仅在担子菌中描述了三种di-TPS。大规模基因组测序计划与合成生物学技术的发展相结合，现在已经能够快速发现和表征来自担子菌真菌的双TPS。本研究采用基因组数据挖掘、系统发育分析和快速产物鉴定相结合的策略，结合合成生物学技术，从220株已测序的担子菌中发现了4个双TPS，并对其进行了功能鉴定。其中，Stereum histurum的SteTC 1为第一个真菌Cembrane二萜合酶; Punctularia strigosozonata的PunTC和Serpula lacryrnans的SerTC为对映贝壳杉烷-16 α-醇合酶; Dentipellis sp的DenTC 3为杯烷合酶。我们的研究结果为通过基因组挖掘发现新的二萜类化合物提供了机会。
Basidiomycete fungi are a rich source of bioactive diterpenoid secondary metabolites. However, compared with the large number of diterpene synthases (di-TPSs) identified in plants and ascomycete fungi, only three di-TPSs have been described from basidiomycete fungi. Large scale genome sequencing projects combined with the development of synthetic biology techniques now has enabled the rapidly discovery and characterization of di-TPSs from basidiomycete fungi. In this study, we discovered and functionally characterized four di-TPSs from 220 genome sequenced basidiomycete fungi by a combined strategy of genomic data mining, phylogenetic analysis and fast products characterization with synthetic biology techniques. Among them, SteTC1 of Stereum histurum was characterized as the first fungal cembrane diterpene synthase; PunTC of Punctularia strigosozonata and SerTC of Serpula lacryrnans were characterized as ent-kauran-16 alpha-ol synthase and DenTC3 of Dentipellis sp was characterized as a cyathane synthase. Our results provide opportunities for the discovery of new diterpenoids from basidiomycete fungi by genome mining.