Advances in genosensor research.
Advances in genosensor research.
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DOI：
10.1093/clinchem/41.5.700
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发表时间：
1995-05
影响因子：
9.3
通讯作者：
K. Beattie;W. Beattie;L. Meng;S. L. Turner;R. Coral-Vázquez;D. D. Smith-D.;P. M. McIntyre;D. D. Dao-D.
中科院分区：
文献类型：
--
作者：
K. Beattie;W. Beattie;L. Meng;S. L. Turner;R. Coral-Vázquez;D. D. Smith-D.;P. M. McIntyre;D. D. Dao-D.
含有核酸杂交位点阵列的微制造装置，称为基因传感器，正在开发用于基因组分析中的各种用途。大量的整体基因传感器的开发工作涉及在基因传感器的实际使用中的实验条件的优化。在这里，我们描述了一种“低技术”形式的基因传感器技术，涉及玻璃显微镜载玻片上的寡核苷酸阵列，它可以用来定义最佳的操作条件，并开发应用程序的杂交阵列在基因组作图和测序。此外，我们描述了一种多孔硅基因传感器，它可以在流通模式下操作，并讨论了它的优势，目前的平面设计。含有DNA片段阵列的多孔硅基因传感器在基因组分析中提供了几种独特的能力。
Microfabricated devices containing arrays of nucleic acid hybridization sites, known as genosensors, are being developed for a variety of uses in genomic analysis. A great deal of the overall genosensor development effort involves optimization of experimental conditions in the actual use of genosensors. Here we describe a "low-tech" form of genosensor technology, involving arrays of oligonucleotides on glass microscope slides, which can be used to define optimal operating conditions and to develop applications of hybridization arrays in genome mapping and sequencing. In addition, we describe a porous silicon genosensor, which can be operated in a flowthrough mode, and discuss its advantages over current flat-surface designs. Porous silicon genosensors containing arrays of DNA fragments offer several unique capabilities in genome analysis.