Expression patterns of two carbonic anhydrase genes, Na+/K+-ATPase and V-type H+-ATPase, in the freshwater crayfish, Cherax quadricarinatus, exposed to low pH and high pH
Expression patterns of two carbonic anhydrase genes, Na+/K+-ATPase and V-type H+-ATPase, in the freshwater crayfish, Cherax quadricarinatus, exposed to low pH and high pH
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DOI：
10.1071/zo16048
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发表时间：
2017-06
影响因子：
0.8
通讯作者：
M. Ali;A. Pavasovic;P. Mather;P. Prentis
中科院分区：
文献类型：
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作者：
M. Ali;A. Pavasovic;P. Mather;P. Prentis
抽象的。碳酸酐酶 (CA)、Na+/K+-ATP 酶 (NKA) 和液泡型 H+-ATP 酶 (HAT) 在十足目甲壳类动物的渗透调节和 pH 平衡中发挥着重要作用。由于 pH 值的变化对甲壳类动物的生理机能有重大影响，因此了解动物维持内部 pH 值的机制至关重要。我们检测了淡水小龙虾鳃中 CA、NKA α 亚基和 HAT 亚基 a 的细胞质 (CAc) 和膜相关形式 (CAg) 在 6.2、7.2（对照）和 8.2 三个 pH 水平（6.2、7.2（对照）和 8.2）24 小时内的表达模式。转移后 24 小时，CAc 的表达水平在低 pH 条件下显着增加，在高 pH 条件下下降。 12小时后，表达在低pH条件下增加，并在24小时时达到最高水平。在低 pH 条件下转移后 6 小时，CAg 的表达显着增加。 NKA 的表达在转移至 pH 6.2 后 6 小时显着增加，并保持升高长达 24 小时。 HAT 和 NKA 的表达显示出相似的模式，其中表达在转移到低 pH 值后 6 小时显着增加，并且在整个实验过程中保持显着升高。总体而言，淡水小龙虾的 CAc、CAg、NKA 和 HAT 基因表达在低 pH 条件下被诱导。
Abstract. Carbonic anhydrase (CA), Na+/K+-ATPase (NKA) and Vacuolar-type H+-ATPase (HAT) play vital roles in osmoregulation and pH balance in decapod crustaceans. As variable pH levels have a significant impact on the physiology of crustaceans, it is crucial to understand the mechanisms by which an animal maintains its internal pH. We examined expression patterns of cytoplasmic (CAc) and membrane-associated form (CAg) of CA, NKA α subunit and HAT subunit a in gills of freshwater crayfish, Cherax quadricarinatus, at three pH levels – 6.2, 7.2 (control) and 8.2 – over 24 h. Expression levels of CAc were significantly increased at low pH and decreased at high pH conditions 24 h after transfer. Expression increased at low pH after 12 h, and reached its maximum level by 24 h. CAg showed a significant increase in expression at 6 h after transfer at low pH. Expression of NKA significantly increased at 6 h after transfer to pH 6.2 and remained elevated for up to 24 h. Expression for HAT and NKA showed similar patterns, where expression significantly increased 6 h after transfer to low pH and remained significantly elevated throughout the experiment. Overall, CAc, CAg, NKA and HAT gene expression is induced at low pH conditions in freshwater crayfish.