Detection of CD44 variants in fine needle aspiration biopsies of thyroid tumor by RT-PCR.
Detection of CD44 variants in fine needle aspiration biopsies of thyroid tumor by RT-PCR.
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通过 RT-PCR 检测甲状腺肿瘤细针抽吸活检中的 CD44 变异。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
N. Amino
中科院分区：
文献类型：
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作者：
Toru Takano;H. Sumizaki;N. Amino
在分化型甲状腺癌中经常观察到CD 44变体的表达增加。为了建立一种不对患者造成额外损伤的甲状腺基因诊断方法，提取细针穿刺活检针内残留细胞的RNA，采用逆转录-聚合酶链反应（RT-PCR）检测CD 44变异体的表达。在FNAB和在载玻片上制备样品用于细胞学检查后，提取针内剩余细胞中的RNA。逆转录后，用CD 44的特异性引物通过PCR扩增cDNA。并与细胞学诊断结果进行比较。CD 44变异体在所有7个被诊断为阳性细胞学的样本中均可检测到。然而，在29个阴性细胞学样本中仅5个检测到它们。因此，甲状腺肿瘤可以更准确地诊断相结合的细胞学检查和检测CD 44变异体的RNA获得FNAB。
Increased expression of CD44 variants is often observed in differentiated thyroid carcinomas. To establish a method for gene diagnosis of thyroid without any additional invasions to the patients, RNA in leftover cells inside the needle used for fine needle aspiration biopsies (FNABs) was extracted to detect the expression of CD44 variants by reverse transcription-polymerase chain reaction (RT-PCR). After FNAB and preparation of sample on a slide glass for cytological examinations, RNA in leftover cells inside the needle was extracted. After reverse transcription, cDNA was amplified by PCR with specific primers for CD44. The results were compared with the cytological diagnosis. CD44 variants were detectable in all seven samples that were diagnosed as positive cytology. However, they were detected in only five of the 29 samples of negative cytology. Therefore, thyroid tumors may be more accurately diagnosed by a combination of cytological examination and detection of CD44 variants in RNA obtained by FNAB.