AN ANTIBODY TO A RECEPTOR FOR FIBRONECTIN AND LAMININ PERTURBS CRANIAL NEURAL CREST DEVELOPMENT INVIVO
AN ANTIBODY TO A RECEPTOR FOR FIBRONECTIN AND LAMININ PERTURBS CRANIAL NEURAL CREST DEVELOPMENT INVIVO
复制标题
DOI：
10.1016/0012-1606(86)90320-9
复制
发表时间：
1986-10-01
影响因子：
2.7
通讯作者：
BRONNERFRASER, M
中科院分区：
文献类型：
--
作者：
BRONNERFRASER, M
本实验室以前的研究（M。Bronner-Fraser（1985）. J. Cell Biol.101，610）已经证明，细胞表面受体复合物的抗体引起鸟类神经嵴细胞迁移的改变。在这里，这些观察被扩展到检查注射抗体的分布和持久性，效果的剂量依赖性，以及抗体注射的长期影响。在颅神经嵴迁移开始时，将识别纤维连接蛋白和层粘连蛋白的细胞表面受体的CSAT抗体注射到中脑神经管的侧面。注射的抗体分子没有穿过中线，但似乎扩散到整个注射的一半的中脑，在那里他们仍然可以检测到免疫细胞化学约22 hr. Embryos进行了检查，无论是在神经嵴迁移（注射后24小时）或神经嵴衍生的结构（注射后36-48小时）形成后。在前24小时内固定的胚胎中，主要缺陷是注射侧神经嵴细胞数量减少，神经管内腔内神经嵴细胞积聚，以及异位定位的神经嵴细胞。在注射后存活36至48小时的胚胎中，神经嵴衍生物在注射侧和对照侧均表现正常，表明胚胎补偿了注射侧神经嵴细胞数量的减少。然而，胚胎往往有严重变形的神经管和异位聚集的神经嵴细胞。相反，几种对照抗体没有效果。这些发现表明，CSAT受体复合物是重要的神经嵴和神经管的正常发育。
Previous studies from this laboratory (M. Bronner-Fraser (1985). J. Cell Biol. 101, 610) have demonstrated that an antibody to a cell surface receptor complex caused alterations in avian neural crest cell migration. Here, these observations are extended to examine the distribution and persistency of injected antibody, the dose dependency of the effect, and the long-term influences of antibody injection. The CSAT antibody, which recognizes a cell surface receptor for fibronectin and laminin, was injected lateral to the mesencephalic neural tube at the onset of cranial neural crest migration. Injected antibody molecules did not cross the midline, but appeared to diffuse throughout the injected half of the mesencephalon, where they remained detectable by immunocytochemistry for about 22 hr. Embryos were examined either during neural crest migration (up to 24 hr after injection) or after formation of neural crest-derived structures (36-48 hr after injection). In those embryo fixed within the first 24 hr, the major defects were a reduction in the neural crest cell number on the injected side, a buildup of neural crest cells within the lumen of the neural tube, and ectopically localized neural crest cells. In embryos allowed to survive for 36 to 48 hr after injection, the neural crest derivatives appeared normal on both the injected and control side, suggesting that the embryos compensated for the reduction in neural crest cell number on the injected side. However, the embryos often had severely deformed neural tubes and ectopic aggregates of neural crest cells. In contrast, several control antibodies had no effect. These findings suggest that the CSAT receptor complex is important in the normal development of the neural crest and neural tube.