A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice.
A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice.
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DOI：
10.1111/nph.13864
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发表时间：
2016-02
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通讯作者：
Muluneh Tamiru;H. Takagi;A. Abe;T. Yokota;H. Kanzaki;H. Okamoto;H. Saitoh;Hideyuki Takahashi;K. Fujisaki;Kaori Oikawa;Aiko Uemura;S. Natsume;Y. Jikumaru;H. Matsuura;K. Umemura;M. J. Terry;R. Terauchi
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作者：
Muluneh Tamiru;H. Takagi;A. Abe;T. Yokota;H. Kanzaki;H. Okamoto;H. Saitoh;Hideyuki Takahashi;K. Fujisaki;Kaori Oikawa;Aiko Uemura;S. Natsume;Y. Jikumaru;H. Matsuura;K. Umemura;M. J. Terry;R. Terauchi
了解植物如何将它们的资源分配到生长或防御上，对于开发不同作物的新品种和改良品种具有长期重要性。利用分子遗传学、植物生理学、激素分析和基于下一代测序(NGS)的转录谱技术，我们分离并鉴定了水稻(Oryza Sativa)的病斑和叶片弯曲(LLB)基因，该基因编码一个以叶绿体为靶标的可能的亮氨酸羧甲基转移酶。LLB功能的丧失会导致生长和产量下降，过敏反应(HR)样病变，抗菌化合物钼内酯和植物胺的积累，以及致病相关基因的结构性表达。与这些防御反应相一致，LLB对稻瘟病(Magnaporthe Oryzae)和白叶枯病(Xanthomonas Oryzae PV)表现出更强的抗性。稻属)。病斑和抗性表型可能是由于茉莉酸(JAs)在LLB突变体中过度积累引起的，其中包括JA前体12-氧代植物二烯酸。此外，由于油菜素类固醇(BR)合成和/或信号的增加，LLB表现出叶片倾斜度增加和早期幼苗生长加速。这些发现表明，LLB在叶绿体中的作用直接或间接地抑制JA和BR介导的反应，揭示了一种可能的机制来控制植物如何分配资源用于防御和生长。
Understanding how plants allocate their resources to growth or defence is of long-term importance to the development of new and improved varieties of different crops. Using molecular genetics, plant physiology, hormone analysis and Next-Generation Sequencing (NGS)-based transcript profiling, we have isolated and characterized the rice (Oryza sativa) LESION AND LAMINA BENDING (LLB) gene that encodes a chloroplast-targeted putative leucine carboxyl methyltransferase. Loss of LLB function results in reduced growth and yield, hypersensitive response (HR)-like lesions, accumulation of the antimicrobial compounds momilactones and phytocassanes, and constitutive expression of pathogenesis-related genes. Consistent with these defence-associated responses, llb shows enhanced resistance to rice blast (Magnaporthe oryzae) and bacterial blight (Xanthomonas oryzae pv. oryzae). The lesion and resistance phenotypes are likely to be caused by the over-accumulation of jasmonates (JAs) in the llb mutant including the JA precursor 12-oxo-phytodienoic acid. Additionally, llb shows an increased lamina inclination and enhanced early seedling growth due to elevated brassinosteroid (BR) synthesis and/or signalling. These findings show that LLB functions in the chloroplast to either directly or indirectly repress both JA- and BR-mediated responses, revealing a possible mechanism for controlling how plants allocate resources for defence and growth.