Chemical-biogeographic survey of secondary metabolism in soil
Chemical-biogeographic survey of secondary metabolism in soil
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DOI：
10.1073/pnas.1318021111
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发表时间：
2014-03-11
影响因子：
11.1
通讯作者：
Brady, Sean F.
中科院分区：
文献类型：
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作者：
Charlop-Powers, Zachary;Owen, Jeremy G.;Brady, Sean F.
在这项研究中，我们比较了美国西南部和东北部地区不同环境中96种土壤微生物的生物合成基因丰富度和多样性。从这些微生物群中扩增的非核糖体肽腺苷酸化（AD）和聚酮酮合酶（KS）结构域片段的454-焦测序为评价不同土壤环境中次生代谢物生物合成多样性的变化提供了一种手段。通过土壤成分和AD-和KS-扩增子丰富度分析，我们确定了土壤类型与升高的生物合成潜力。一般而言，干旱土壤显示最丰富的观察到的生物合成多样性，而半咸水沉积物和松林土壤显示最少。通过映射单个环境扩增子序列的序列来源于功能特征的生物合成基因簇，我们确定了保守的土壤类型特定的次级代谢物富集模式，尽管显着的样品间序列变异。这些数据用于创建环境中具有生物医学价值的天然产物家族的化学生物地理分布图，这些分布图应被证明对指导未来生物活性天然产物的发现是有用的。
In this study, we compare biosynthetic gene richness and diversity of 96 soil microbiomes from diverse environments found throughout the southwestern and northeastern regions of the United States. The 454-pyroseqencing of nonribosomal peptide adenylation (AD) and polyketide ketosynthase (KS) domain fragments amplified from these microbiomes provide a means to evaluate the variation of secondary metabolite biosynthetic diversity in different soil environments. Through soil composition and AD-and KS-amplicon richness analysis, we identify soil types with elevated biosynthetic potential. In general, arid soils show the richest observed biosynthetic diversity, whereas brackish sediments and pine forest soils show the least. By mapping individual environmental amplicon sequences to sequences derived from functionally characterized biosynthetic gene clusters, we identified conserved soil type-specific secondary metabolome enrichment patterns despite significant sample-to-sample sequence variation. These data are used to create chemical biogeographic distribution maps for biomedically valuable families of natural products in the environment that should prove useful for directing the discovery of bioactive natural products in the future.