Nestin-Expressing Interfollicular Blood Vessel Network Contributes to Skin Transplant Survival and Wound Healing
Nestin-Expressing Interfollicular Blood Vessel Network Contributes to Skin Transplant Survival and Wound Healing
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DOI：
10.1002/jcb.22512
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发表时间：
2010-05-01
影响因子：
4
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
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作者：
Aki, Ryoichi;Amoh, Yasuyuki;Hoffman, Robert M.
使用巢蛋白驱动的绿色荧光蛋白（ND-GFP）转基因小鼠，我们以前证明了一个毛囊间血管网络，表达ND-GFP，似乎起源于ND-GFP表达毛囊干细胞。我们在这里报告说，移植皮肤或愈合伤口的血管生成起源于这个表达ND-GFP的微血管网络。在皮肤移植或伤口愈合后，可见表达ND-GFP的血管从表达ND-GFP的毛囊干细胞区域生长并重建真皮微血管网络。当将来自ND-GFP小鼠的触须（触须）的ND-GFP干细胞区域移植到普遍表达RFP的转基因小鼠时，我们观察到嵌合的ND-GFP-RFP血管，这表明来自移植物和宿主的滤泡间血管网络的连接。这些观察结果表明，毛囊间血管网络有助于皮肤移植存活和伤口愈合。J.细胞。110：80-86，2010. (C)2010 Wiley-Liss，Inc.
Using nestin-driven green fluorescent protein (ND-GFP) transgenic mice, we previously demonstrated an inter-hair-follicle blood vessel network that expresses ND-GFP and appears to originate from ND-GFP expressing hair-follicle stem cells. We report here that angiogenesis of transplanted skin or healing wounds originates from this ND-GFP-expressing microvasculature network. ND-GFP-expressing blood vessels were visualized growing from the ND-GFP-expressing hair-follicle stem cell area and re-establishing the dermal microvasculature network after skin transplantation or wound healing. When the ND-GFP stem cell area from the vibrissa (whisker) from ND-GFP mice was transplanted to transgenic mice ubiquitously expressing RFP, we observed chimeric ND-GFP-RFP blood vessels, suggesting the joining of inter-follicular blood vessel networks from the transplant and host. These observations suggest that the inter-hair-follicle blood-vessel network contributes to skin transplant survival and wound healing. J. Cell. Biochem. 110: 80-86, 2010. (C) 2010 Wiley-Liss, Inc.