Effect of corticosteroids on brain growth in fetal sheep
Effect of corticosteroids on brain growth in fetal sheep
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DOI：
10.1016/s0029-7844(99)00265-3
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发表时间：
1999-08-01
影响因子：
7.2
通讯作者：
Dunlop, SA
中科院分区：
文献类型：
--
作者：
Huang, WL;Beazley, LD;Dunlop, SA
目的：比较单次和多次皮质类固醇激素对胎羊脑发育的影响。方法：孕105天的绵羊肌肉注射betteramine（0.5mg/kg），111、118和124天分别注射等量生理盐水（n = 12）和betteramine（n = 12）。对照组在所有四个时间间隔接受等体积的无菌生理盐水（n = 12）。羔羊在125天（早产）或145天（足月）分娩。灌注后，我们测量了全脑、大脑、小脑和脑干的重量（克）、体积全脑和大脑的最大长度、宽度和深度（毫升）结果：在早产时单次注射组中，无显著差异（P = 0.070）皮质类固醇治疗动物（38.0 +/- 1.81 g）和对照组（42.5 +/- 1.65 g）之间的全脑重量。皮质类固醇组的大脑长度和深度显著减少（P <0.05）;其他指标无显著差异。足月时，全脑重量（47.5 +/- 1.70 g; P = 0.022）显著低于对照组（53.4 +/- 1.73 g）。除大脑和脑干重量以及大脑长度外，所有其他测量值均显著降低（P <0.05）。在早产时接受重复注射的组中，与对照组（42.5 +/-1.65 g）相比，全脑重量显著降低（35.5 +/-1.65 g; P = 0.005）。除小脑和脑干重量外，所有其他指标均显着降低（P < .05）。足月时，与对照组（53.4 +/- 1.73 g）相比，全脑重量也显著降低（42.4 +/- 1.52 g; P =.001），所有其他指标也是如此（P < .05）。(C)1999年由美国产科医生和犬牙学家学院。
Objective: To compare the effects of single and repeated courses of corticosteroids on brain growth in fetal sheep.Methods: Pregnant sheep were given intramuscular betamethasone (0.5 mg/kg) at 105 days' gestation followed at 111, 118, and 124 days by equivalent volumes of sterile normal saline (n = 12) or betamethasone (n = 12). Controls received equivalent volumes of sterile normal saline at all four intervals (n = 12). Lambs were delivered at 125 (preterm) or 145 (term) days. After perfusion, we measured weights (grams) for whole brain, cerebrum, cerebellum and brain stem, volumes (milliliters) for whole brain and cerebrum, and maximum cerebral anterior-posterior length, width, and depth (centimeters).Results: In the single-injection group at preterm, there were no significant differences (P =.070) in whole-brain weight between the corticosteroid-treated animals (38.0 +/- 1.81 g) and controls (42.5 +/- 1.65 g). Cerebral length and depth were significantly reduced in the corticosteroid group (P < .05); other measures were not significantly different. At term, whole-brain weight was significantly lower (47.5 +/- 1.70 g; P = .022) compared with controls (53.4 +/- 1.73 g). All other measures were significantly reduced (P < .05) except cerebral and brain-stem weights and cerebral length. In the group that received repeated injections at preterm, whole-brain weight was significantly reduced (35.5 +/- 1.65 g; P = .005) compared with controls (42.5 +/- 1.65 g). All other measures were significantly reduced (P < .05) except cerebellar and brain-stem weights. At term, whole-brain weight was also significantly reduced (42.4 +/- 1.52 g; P =.001) compared with controls (53.4 +/- 1.73 g) as were all other measures (P < .05).Conclusion: Administration of single and repeated courses of corticosteroids to pregnant sheep retarded fetal braingrowth. (C) 1999 by The American College of Obstetricians and Cyneeologists.